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木聚糖酶高产菌株的诱变*
引用本文:陈红歌, 刘新育, 张世敏, 宋安东, 贾新成,.木聚糖酶高产菌株的诱变*[J].微生物学通报,2004,31(6):33-36.
作者姓名:陈红歌  刘新育  张世敏  宋安东  贾新成  
作者单位:河南农业大学生物技术与食品科学学院,郑州,450002
基金项目:河南省科技厅科技攻关资助项目 (No 0 2 2 40 1 0 0 0 5 )
摘    要:出发菌株Aspergillus niger M1经过紫外线诱变得到一株木聚糖酶活力提高30%的突变株A.niger J506。木聚糖酶谱带检测发现,突变株成熟发酵液中有3种类型的木聚糖酶,而出发菌株中只有两种。经过正交试验得出突变株产酶的最佳发酵条件为:主碳源浓度4%、麸皮与玉米芯的比例为5:5、葡萄糖浓度0.1%、草酸铵浓度2.0%,培养基初始pH为5.0,250mL三角瓶的装液量为100mL。

关 键 词:木聚糖酶  诱变
文章编号:0253-2654(2004)06-0033-04
修稿时间:2004年1月5日

Mutation of the Strain Producing Higher Xylanase
Abstract:A.niger M1, the initial strain, was treated by UV and a mutant with 30% higher xylanase activity was obtained. Zymogram for detecting xylanase showed there are three different xylanases in the mutant mature culture, while two xylanases in initial strain. After orthogonal experiment, the optimum fermentation conditions of the mutant were obtained as follows: concentration of the major carbon resource 4 %, ratio between bran and corncob 5:5, concentration of glucose 0.1%, concentration of ammonium oxalate as supplemental nitrogen resource 2.0%, the initial pH of liquid medium 5.0, 100mL/250mL flask.
Keywords:Xylanase  Mutation
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