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Biolog EcoPlateTM实验信息提取方法改进
引用本文:张燕燕,曲来叶,陈利顶.Biolog EcoPlateTM实验信息提取方法改进[J].微生物学通报,2009,36(7):1083-1091.
作者姓名:张燕燕  曲来叶  陈利顶
作者单位:1. 中国科学院生态环境研究中心,城市与区域生态国家重点实验室,北京,100085;中国科学院研究生院,北京,100049
2. 中国科学院生态环境研究中心,城市与区域生态国家重点实验室,北京,100085
基金项目:国家“十一五”科技支撑计划课题(No. 2006BAC10B05)
摘    要:Biolog EcoPlateTM微平板是用于研究微生物群落水平多样性的一种主流研究方法。针对目前国内研究者对Biolog实验结果解读不充分和错误层出不穷的情况, 提出了一套改进实验结果信息提取的方法。方法包含两个部分, 一是基于微生物生理学和生态学意义上的Biolog碳源分类表, 是研究者进行解读的参考依据和索引; 二是结合实例, 阐释如何根据上述分类表、以环境富集原理为指导, 利用排序的统计方法进行结果解译, 发掘出更多的信息。

关 键 词:Biolog    群落水平多样性    碳源    分类    解读

An Amendment on Information Extraction of Biolog EcoPlateTM
ZHANG Yan-Yan,QU Lai-Ye and CHEN Li-Ding.An Amendment on Information Extraction of Biolog EcoPlateTM[J].Microbiology,2009,36(7):1083-1091.
Authors:ZHANG Yan-Yan  QU Lai-Ye and CHEN Li-Ding
Affiliation:(1. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China) (2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China);State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
Abstract:Biolog EcoPlateTM is a mainstream approach of studying community-level physiological profiles of microbes. To correct misunderstanding and insufficiency of experimental result explanation, we announced an amendment of information extraction of Biolog EcoPlateTM, which included two parts: (1) carbon substrates assortment sheets based on microbial physiological metabolism and ecological function, providing referrence and index for researchers; (2) illustration of how to use assorment sheet and ordination method to find out more information, guiding by environmental concentration principle.
Keywords:Biolog EcoPlate  Community-level physiological profiles  Carbon substrates  Classification  Explanation
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