首页 | 官方网站   微博 | 高级检索  
     

药用植物内生放线菌的分离、筛选及活性菌株YIM 61470鉴定
引用本文:秦盛,赵立兴,陈云,赵国振,李洁,朱文勇,黄海玉,徐丽华.药用植物内生放线菌的分离、筛选及活性菌株YIM 61470鉴定[J].微生物学通报,2009,36(11):1693-1699.
作者姓名:秦盛  赵立兴  陈云  赵国振  李洁  朱文勇  黄海玉  徐丽华
作者单位:1. 云南大学,云南省微生物研究所,云南省生物资源保护与利用重点实验室,云南,昆明,650091;徐州师范大学,江苏省药用植物生物技术重点实验室,江苏,徐州,221116
2. 云南大学,云南省微生物研究所,云南省生物资源保护与利用重点实验室,云南,昆明,650091
基金项目:国家重大基础研究发展规划项目(No. 2004CB719601); 国家科技部国际合作项目(No. 2006DFA33550); 云南省国际合作项目(No. 2005GH21); 云南省教育厅科学研究基金项目(No. 08J0008)
摘    要:从云南西双版纳热带雨林多种药用植物中分离到272株内生放线菌,活性筛选表明 146株菌的发酵产物具有抗菌活性,其中94株菌具有拮抗病原细菌活性,127株菌具有抑制病原真菌的功能.分离菌株YIM 61470具有广谱抗菌活性,通过形态特征、培养特征、生理生化特征、细胞化学分类特征和基于16S rRNA基因序列的相似性分析等研究,菌株YIM 61470被鉴定为链霉菌属(Streptomyces)氢化链霉菌(S.llydrogenans)的一个菌株.

关 键 词:药用植物  内生放线菌  抗菌活性  鉴定  16S  rRNA基因

Endophytic Actinomycetes Associated with Medicinal Plants: Isolation, Antimicribial Activity and Identification
QIN Sheng,ZHAO Li-Xing,CHEN Yun,ZHAO Guo-Zhen,LI Jie,ZHU Wen-Yong,HUANG Hai-Yu and XU Li-Hua.Endophytic Actinomycetes Associated with Medicinal Plants: Isolation, Antimicribial Activity and Identification[J].Microbiology,2009,36(11):1693-1699.
Authors:QIN Sheng  ZHAO Li-Xing  CHEN Yun  ZHAO Guo-Zhen  LI Jie  ZHU Wen-Yong  HUANG Hai-Yu and XU Li-Hua
Affiliation:Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunan 650091, China; Key Laboratory of Biotechnology for Me;Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunan 650091, China;Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunan 650091, China;Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunan 650091, China;Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunan 650091, China;Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunan 650091, China;Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunan 650091, China;Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunan 650091, China
Abstract:272 endophytic actinomycetes were isolated from different medicinal plants collected from tropical rainforest in Xishuangbanna of Yunnan province. By antimicrobial screening, the fermentation broth of 146 strains inhibited the tested pathogenetic strains, and 94 or 127 out of the 272 endophytic actinomycetes strains showed inhibition activity against pathogenetic bacteria or fungi. The strain YIM 61470 exhibited extensive antagonism against pathogenic microbes. Based on the morphological characteristics, cultural characteristics, physiological characteristics, chemotaxonomic data and 16S rRNA gene sequence similarity analysis, strain YIM 61470 was identified as Streptomyces hydrogenans, a known species of the genus Streptomyces.
Keywords:Medicinal plants  Endophytic actinomycetes  Antimicrobial activity  Identification  16S rRNA gene
本文献已被 万方数据 等数据库收录!
点击此处可从《微生物学通报》浏览原始摘要信息
点击此处可从《微生物学通报》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司    京ICP备09084417号-23

京公网安备 11010802026262号