| 应用分散和差速离心法分离嗜酸和耐酸链霉菌的试验及评价* |
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| 引用本文： | 王黎明, 黄英, 崔庆锋, 谢琼, 张亚美, 刘志恒,.应用分散和差速离心法分离嗜酸和耐酸链霉菌的试验及评价*[J].微生物学通报,2003,30(6):81-86. |
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| 作者姓名： | 王黎明 黄英 崔庆锋 谢琼 张亚美 刘志恒 |
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| 作者单位： | 中国科学院微生物研究所国家微生物资源重点实验室,北京,100080 |
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| 基金项目： | 国家自然科学基金(No.30270003)中-英合作研究项目(Q814) |
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| 摘 要： | 新的微生物资源的开发离不开菌种分离技术的改进和分离效率的提高。我们采用一种新的分离方法——分散和差速离心法(Dispersion and Differential Centrifugation，DDC)，以传统的振荡法做对照，对12份酸性土壤样品进行了链霉菌的分离。结果表明DDC方法的分离效率是传统方法的2—20倍，且选择性好。用DDC方法共分离出链霉菌249株，归属于12个颜色类群，其中的45株代表菌株的形态和细胞壁类型均符合链霉菌的特征，最适生长pH均为4．5—5．5。结果表明应用DDC方法分离非常见的嗜酸和耐酸链霉菌是可行的。
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| 关 键 词： | 分散和差速离心 嗜酸链霉菌 耐酸链霉菌 分离 |
| 文章编号： | 0253-2654(2003)06-0081-06 |
| 修稿时间： | 2003年2月21日 |
ISOLATION OF ACIDOPHILIC AND ACIDODURIC STREPTOMYCETES USING DISPERSION AND DIFFERENTIAL CENTRIFUGATION APPROACH |
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| WANG Li-Ming HUANG Ying CUI Qing-Feng XIE Qiong ZHANG Ya-Mei LIU Zhi-Heng.ISOLATION OF ACIDOPHILIC AND ACIDODURIC STREPTOMYCETES USING DISPERSION AND DIFFERENTIAL CENTRIFUGATION APPROACH[J].Microbiology,2003,30(6):81-86. |
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| Authors: | WANG Li-Ming HUANG Ying CUI Qing-Feng XIE Qiong ZHANG Ya-Mei LIU Zhi-Heng |
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| Abstract: | Technological improvement for microorgnism isolation is important since isolation provides substantial materials for the exploitation of new microbial resources. In this study, a new approach, dispersion and differential cetrifugation (DDC), was applied in the isolation of acidophilic and acidoduric streptomycetes from 12 acid soil samples. Contrast with traditional method, the new approach yielded satisfying results with 2 - 20 times isolation efficiency and good selectivity. 45 representatives out of 249 streptomycetes isolates, which belonged to 12 color groups, showed morphology and cell wall type consistent with streptomycetes. The optimum pH range for their growth were between pH 4.5 - 5.5. It is proved that we succeeded in the rare-streptomycetes isolation using DDC approach. |
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| Keywords: | Dispersion and differential centrifugation Acidophilic streptomycetes Acidoduric streptomycetes Isolation |
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