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添加核苷对肝素黄杆菌发酵产肝素酶的影响
引用本文:马小来, 顾宇翔, 袁勤生,.添加核苷对肝素黄杆菌发酵产肝素酶的影响[J].微生物学通报,2005,32(5):92-97.
作者姓名:马小来  顾宇翔  袁勤生  
作者单位:华东理工大学生物反应器工程国家重点实验室,上海,200237
摘    要:研究了添加核苷对肝素黄杆菌发酵产肝素酶的影响,结果发现,单种核苷的添加会抑制产酶,而复合核苷的添加则促进产酶,当4种核苷的添加比例与肝素酶mRNA中4种相应核苷酸的比例一致时,促进作用最强。通过HPLC检测,证实添加后核苷很快进入了菌体内。HPLC的结果还表明,菌体内嘧啶核苷酸和嘌呤核苷酸的合成代谢可能不平衡,这对产酶是不利的。为此,还研究了通过添加天冬氨酸以增强嘧啶核酸合成代谢的调节方式,使产酶得到了提高。

关 键 词:核苷  肝素黄杆菌  肝素酶
文章编号:0253-2654(2005)05-0092-06
收稿时间:12 9 2004 12:00AM
修稿时间:01 31 2005 12:00AM

Effect of Addition Nucleotides on Heparinase Production by Flavobacterium heparinum
MA Xiao-Lai,GU Yu-Xiang,YUAN Qin-Sheng.Effect of Addition Nucleotides on Heparinase Production by Flavobacterium heparinum[J].Microbiology,2005,32(5):92-97.
Authors:MA Xiao-Lai  GU Yu-Xiang  YUAN Qin-Sheng
Abstract:The effect of addition nucleotides on heparinase production by Flavobacterium heparinum was reseached. The result indicated that addition of nucleotides cause different effects on heparinase production depend on its addi ng mode, single kind of nucleotide would restrain, but multiplex promote. The s trongest promotion happened when the propotion of nucleotides was consistent wit h the nucleic acids of heparinase mRNA. HPLC result confined the entrance of nu cleoides to the bactera cell. And it also suggested the synthesis metabolic of nucleic acids in F.heparinum was imbalance, there always be higher lever of purin nucleoides. L-Asp was added to medium culture to enhance pyridine nucle oides thynesis in order to improve the enzyme production ,which has been affecte d by the imbalance of nucleoides.
Keywords:Nucleotides  Flavobacterium heparinum  Heparinase
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