| 产harpin的成团泛菌工程菌308R（pCPP430）遗传稳定性的研究 |
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| 引用本文： | 李艳琴,宁红秀.产harpin的成团泛菌工程菌308R（pCPP430）遗传稳定性的研究[J].微生物学通报,1999,26(6):400-403. |
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| 作者姓名： | 李艳琴 宁红秀 |
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| 作者单位： | 山西大学生物工程开放实验室!太原030006 (李艳琴，宁红秀，申泉，赵春贵，李新锋)，山西大学生物工程开放实验室!太原030006(赵立平) |
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| 基金项目： | 国家高技术研究发展计划项目（“863”项目），Project of Chinese National Programs for |
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| 摘 要： | 成团泛菌工程菌３０８Ｒ（ｐＣＰＰ４３０）带有梨火疫欧文氏杆菌的与过敏反应和致病性有关的基因簇 （ｈｒｐ），可以产生能诱导植物抗病性的蛋白质ｈａｒｐｉｎ。该工程菌在ＬＢ液体培养基中生长５０代后，带有重 组质粒ｐＣＰＰ４３０的细胞占总菌量的 １％，带有载体ｐＣＰＰ９的细胞为４６％。工程菌喷雾到番茄叶面，保湿条 件下叶面菌量维持在 １０５ｃｆｕ／ｃｍ２以上，其中带有 ｐＣＰＰ４３０质粒的菌维持在 ４０％以上，带有 ｐＣＰＰ９载体的 菌维持在８０％以上。因此，携带ｈｒｐ基因簇的质粒ｐＣＰＰ４３０在宿主菌中是不稳定的。文中讨论了改进工 程菌遗传稳定性的途径。
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| 关 键 词： | 工程菌 质粒 遗传稳定性 诱导抗性 成团泛菌 |
GENETIC STABILITY OF A RECOMBINANT PANTOEA AGGLOMERANS STRAIN 308R(pCPP430) CAPABLE OF PRODUCING PLANT RESISTANCE INDUCING PROTEIN HARPIN |
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| LI Yanqin,NING Hongxiu,SHEN Quan,ZHAO Chungui,LI Xinfeng,ZHAO Liping.GENETIC STABILITY OF A RECOMBINANT PANTOEA AGGLOMERANS STRAIN 308R(pCPP430) CAPABLE OF PRODUCING PLANT RESISTANCE INDUCING PROTEIN HARPIN[J].Microbiology,1999,26(6):400-403. |
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| Authors: | LI Yanqin NING Hongxiu SHEN Quan ZHAO Chungui LI Xinfeng ZHAO Liping |
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| Affiliation: | Shanxi University Shanxi Key Laboratory of Biotechnology Taiyuan 030006 |
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| Abstract: | The genetic stability of Pantoea agglomerans 308R(pCPP430), a recombinant strain capable of producing the plant resistance inducing protein harpin, was studied both in medium and in plant under antibiotic-free conditions. After successive growth in LB medium for 50 generations, only 1% of the cells still retained the plasmid pCPP430, in comparison with 46% of the vector pCPP9. After sprayed onto the leaves of tomato, the recombinant strain maintained a population density of 10~5cfu /cm2 when kept under high humidity. 40% cells of this population contained the plasmid pCPP430. It was shown that plasmid pCPP430 was unstable due to inadequate partition. Strategies for improving its genetic stability were discussed. |
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| Keywords: | Recombinant bacteria Plasmid Genetic stability Resistance inducing Pantoea agglomerans |
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