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过量无机盐及谷氨酸抑制重组菌核黄素发酵
引用本文:司马迎春, 班睿,.过量无机盐及谷氨酸抑制重组菌核黄素发酵[J].微生物学通报,2006,33(2):86-89.
作者姓名:司马迎春  班睿  
作者单位:天津大学化工学院生物工程系,天津,300072
摘    要:在重组枯草芽孢杆菌24/pMX45核黄素发酵中,酵母粉促进核黄素合成,酵母抽提物抑制核黄素合成。分析显示,酵母抽提物的无机离子和游离氨基酸含量均高于酵母粉。在酵母粉基础发酵培养基中,添加各种无机离子和游离氨基酸,使其含量与酵母抽提物相同。摇瓶发酵结果表明:过量的无机离子和谷氨酸对核黄素合成有显著的抑制作用。酵母抽提物含有较高浓度的谷氨酸,是其抑制核黄素合成的主要原因。

关 键 词:酵母粉  酵母抽提物  发酵  核黄素  重组枯草芽孢杆菌
文章编号:0253-2654(2006)02-0086-04
收稿时间:2005-07-04
修稿时间:2005-08-24

Excess Inorganic Salt and Glutamine Repress Riboflavin Fermentation of Recombinant Bacillus subtilis
SIMA Ying-Chun,BAN Rui.Excess Inorganic Salt and Glutamine Repress Riboflavin Fermentation of Recombinant Bacillus subtilis[J].Microbiology,2006,33(2):86-89.
Authors:SIMA Ying-Chun  BAN Rui
Abstract:In the riboflavin fermentation by recombinant Bacillus subtilis 24/pMX45,The yeast powder facilitate riboflavin biosynthesis and the yeast extract have negative effect. It was found that the concentrations of inorganic ions and the amino acids in yeast extract were much higher than that of the yeast powder. The inorganic ions and the amino acids were respectively added to the medium while yeast powder was used as base nitrogen source. The fermentation results show that excess inorganic ions and glutamine have negative effect on riboflavin biosynthesis. The yeast extract restrained riboflavin biosynthesis for it contains much glutamic acid.
Keywords:Yeast powder  Yeast extract  Fermentation  Riboflavin  Recombinant Bacillus subtlis  
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