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随机扩增多态DNA(RAPD)在野蘑菇杂交育种中的应用
引用本文:李荣春, 张丽梅,.随机扩增多态DNA(RAPD)在野蘑菇杂交育种中的应用[J].微生物学通报,2001,28(5):52-55.
作者姓名:李荣春  张丽梅  
作者单位:云南农业大学食用菌研究所
摘    要:野蘑菇 (AgaricusarvensisSchaeffex .Fr.)是蘑菇属 (Agaricus)又一种近年来广泛栽培的食用菌。由于它的菌丝细胞具有多核 ,无锁状联合 ,以及人们对它的繁殖模式和生活史认识的不足 ,给杂交育种工作造成了较多困难。运用随机扩增多态DNA遗传标记技术 ,结合拮抗试验和核相分析 ,对自育的单孢菌株之间的杂交试验进行分析研究。结果表明 :两个相互亲合的同核体菌株被配对培养时 ,交配反应出现 ,并形成异核体的后代。可能野蘑菇具有双重的交配繁殖系统———

关 键 词:RAPD    野蘑菇    杂交育种  
文章编号:0253-2654(2001)05-0052-04
修稿时间:2000年4月15日

RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) REVEALED THE HETEROKARYON OF AGARICUS ARVENSIS PRODUCED BY MATING REACTIONS
LI Rong-Chun,ZHAN Li-Mei.RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) REVEALED THE HETEROKARYON OF AGARICUS ARVENSIS PRODUCED BY MATING REACTIONS[J].Microbiology,2001,28(5):52-55.
Authors:LI Rong-Chun  ZHAN Li-Mei
Abstract:s: The horse mushroom (Agaricus arvensis Schaeff ex Fr.) is one of most excellent edible mushroom,and was cultivated in world at resent year.But up to now,the breeding systems and life-cycle of horse mushroom has been poorly know.In this study,the random amplified polymorphic DNA was employed to analyse the mating reaction between self-fertile homokaryonic isolates by paired.The results demonstrated: (1) the mating reaction happened and the heterokaryonic offspring formed when the intercompatible self-fertile homokaryonic isolates were paired;(2) perhaps the horse mushroom is possessed of double breeding systems-homothallism and heterothallism;(3) RAPD is very effective method for verifying artificially synthesized heterokaryons and monitoring the transmission of genetic loci in mating of fungi.
Keywords:RAPD  Agaricus arvensis  Mating reaction    
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