Fatty acid lithium salts from Cunninghamella echinulata have cytotoxic and genotoxic effects on HL‐60 human leukemia cells |
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| Authors: | Raghda Alakhras Stamatia Bellou Grammatiki Fotaki Georgia Stephanou Nikos A Demopoulos Seraphim Papanikolaou George Aggelis |
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| Affiliation: | 1. Division of Genetics, Cell and Developmental Biology, Department of Biology, University of Patras, Patras, Greece;2. Department of Food Science and Human Nutrition, Agricultural University of Athens, Athens, Greece;3. Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia |
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| Abstract: | Polyunsaturated fatty acids, especially gamma linolenic acid (GLA), are potentially useful agents in the treatment of cancer. Cunninghamella echinulata, a fungus species that is able to synthesize GLA, when cultivated under nitrogen‐limited conditions in a medium having glucose as carbon and energy source, accumulated 32–35% of lipids containing 11–18% GLA. The conversion yield of glucose to lipid was around 0.11 g per gram of glucose consumed while the lipid production was 5 g/L. Fatty acid lithium salts (FALS) were prepared from the total Cunninghamella lipids and studied for their effects on HL‐60 human leukemic cells. Cytotoxicity of FALS on HL‐60 leukemic cells was linearly related to the FALS concentration. High FALS concentration (i.e. 15 and 20 μg/mL) induced DNA fragmentation, while concurrent treatment of cells with H2O2 (at 100 μM) and FALS resulted in enhanced cytotoxicity of H2O2. However, when FALS were employed at low concentrations (i.e. 5 and 10 μg/mL), they demonstrated a protective effect on HL‐60 cells against H2O2 genotoxicity, whereas at 20 μg/mL FALS enhanced the ability of H2O2 to induce DNA fragmentation. It is concluded that FALS derived from C. echinulata lipids could be an effective preparation against HL‐60 human leukemic cells. |
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| Keywords: | Cytotoxicity Gamma linolenic acid Genotoxicity HL‐60 leukemic cells Lipid accumulation |
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