OsDi19‐4 acts downstream of OsCDPK14 to positively regulate ABA response in rice |
| |
| Authors: | Lili Wang Changchun Yu Shanglin Xu Yingguo Zhu Wenchao Huang |
| |
| Affiliation: | 1. State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China;2. Key Laboratory for Research and Utilization of Heterosis in Indica Rice, the Ministry of Agriculture, The Yangtze River Valley Hybrid Rice Collaboration & Innovation Center, Wuhan University, Wuhan, China |
| |
| Abstract: | The drought‐induced 19 protein family consists of several atypical Cys2/His2‐type zinc finger proteins in plants and plays an important role in abiotic stress. In this study, we found that overexpressing OsDi19‐4 in rice altered the expression of a series of abscisic acid (ABA)‐responsive genes, resulting in strong ABA‐hypersensitive phenotypes including ABA‐induced seed germination inhibition, early seedling growth inhibition and stomatal closure. On the contrary, OsDi19‐4 knockdown lines were less sensitive to ABA. Additionally, OsCDPK14 was identified to interact with OsDi19‐4 and be responsible for the phosphorylation of OsDi19‐4, and the phosphorylation of OsDi19‐4 was further enhanced after the treatment of ABA. Apart from these, OsDi19‐4 was shown to directly bind to the promoters of OsASPG1 and OsNAC18 genes, two ABA‐responsive genes, and regulate their expression. Transient expression assays confirmed the direct regulation role of OsDi19‐4, and the regulation was further enhanced by the increased phosphorylation of OsDi19‐4 after the treatment of ABA. Taken together, these data demonstrate that OsDi19‐4 acts downstream of OsCDPK14 to positively regulate ABA response by modulating the expression of ABA‐responsive genes in rice. |
| |
| Keywords: | Di19 ABA signal CDPK phosphorylation stress responses |
|
|
