Activation of a Bean Chitinase Promoter in Transgenic Tobacco Plants by Phytopathogenic Fungi |
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| Authors: | Roby D Broglie K Cressman R Biddle P Chet I L Broglie R |
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| Affiliation: | E.I. Du Pont de Nemours & Company, Agricultural Products Department, Experimental Station, P.O. Box 80402, Wilmington, Delaware 19880. |
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| Abstract: | The temporal and spatial expression of a bean chitinase promoter has been investigated in response to fungal attack. Analysis of transgenic tobacco plants containing a chimeric gene composed of a 1.7-kilobase fragment carrying the chitinase 5B gene promoter fused to the coding region of the gus A gene indicated that the chitinase promoter is activated during attack by the fungal pathogens Botrytis cinerea, Rhizoctonia solani, and Sclerotium rolfsii. Although induction of beta]-glucuronidase activity was observed in tissues that had not been exposed to these phytopathogens, the greatest induction occurred in and around the site of fungal infection. The increase in beta]-glucuronidase activity closely paralleled the increase in endogenous tobacco chitinase activity produced in response to fungal infection. Thus, the chitinase 5B-gus A fusion gene may be used to analyze the cellular and molecular details of the activation of the host defense system during pathogen attack. |
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