Both internal and external regulatory elements control expression of the pea Fed-1 gene in transgenic tobacco seedlings. |
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| Authors: | M Gallo-Meagher D A Sowinski R C Elliott and W F Thompson |
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| Affiliation: | Department of Genetics, North Carolina State University, Raleigh 27695. |
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| Abstract: | In previous studies using leaves of light-grown transgenic tobacco plants, we have shown that sequences located within the transcribed region of the pea Fed-1 gene (encoding ferredoxin I) are major cis-acting determinants of light-regulated mRNA accumulation. However, we show here that these internal sequences are less important for the Fed-1 light response in etiolated tobacco seedlings than they are in green leaves and that upstream elements confer organ specificity and contribute significantly to Fed-1 light responses in etiolated material. Light effects mediated by upstream response elements are thus most pronounced during the initial induction of gene activity, whereas internal elements play a more prominent role in modulating Fed-1 expression once the gene is already active. |
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