Phosphorylation of ribosomal proteins induced by auxins in maize embryonic tissues |
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| Authors: | Pérez L Aguilar R Méndez A P de Jiménez E S |
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| Affiliation: | Departamento de Bioquimica, Facultad de Quimica, U.N.A.M. México, D.F. 04510. |
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| Abstract: | The effect of auxin on ribosomal protein phosphorylation of germinating maize (Zea mays) tissues was investigated. Two-dimensional gel electrophoresis and autoradiography of 32P] ribosomal protein patterns for natural and synthetic auxin-treated tissues were performed. Both the rate of 32P incorporation and the electrophoretic patterns were dependent on 32P pulse length, suggesting that active protein phosphorylation-dephosphorylation occurred in small and large subunit proteins, in control as well as in auxin-treated tissues. The effect of ribosomal protein phosphorylation on in vitro translation was tested. Measurements of poly(U) translation rates as a function of ribosome concentration provided apparent Km values significantly different for auxin-treated and nontreated tissues. These findings suggest that auxin might exert some kind of translational control by regulating the phosphorylated status of ribosomal proteins. |
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