The effect of low osmotic potential on nitrite reduction in intact spinach chloroplasts |
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| Authors: | Behrens P W Xu F Werner M Hoffman T Marsho T V Mackay A B |
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| Affiliation: | Department of Biological Sciences, University of Maryland Baltimore County (U.M.B.C.), Catonsville, Maryland 21228. |
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| Abstract: | The effect of water stress (reduced osmotic potential) on photosynthetic nitrite reduction was investigated using intact, isolated spinach (Spinacia oleracea) chloroplasts. Nitrite-dependent O2 evolution was inhibited 39% at −29.5 bars osmotic potential, relative to a control at −11 bars. In the presence of an uncoupler of photophosphorylation this inhibition was not seen. Reduced osmotic potential did not inhibit either methyl viologen reduction or photosynthetic O2 reduction. These results indicate that an inhibition of electron transport to ferredoxin cannot account for the observed inhibition of nitrite-dependent O2 evolution. In vitro assay of nitrite reductase activity showed that the interaction of the enzyme with nitrite was not affected by changes in the concentrations of ions or molecules that might be caused by water stress conditions. These results indicate that the most likely site for the effect of water stress on chloroplastic nitrite reduction is the interaction of ferredoxin with nitrite reductase. |
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