| 基于cDNA芯片的梨品种S基因型鉴定及新S-RNase基因进化分析 |
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| 引用本文： | 江南,张琳,谭晓风,谭慧,张靖国.基于cDNA芯片的梨品种S基因型鉴定及新S-RNase基因进化分析[J].植物遗传资源学报,2017,18(3):520-529. |
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| 作者姓名： | 江南 张琳 谭晓风 谭慧 张靖国 |
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| 作者单位： | 1. 湖南工业大学包装与材料工程学院,株洲412007;中南林业科技大学经济林培育与保护教育部重点实验室/经济林育种与栽培国家林业局重点实验室,长沙410004;2. 中南林业科技大学经济林培育与保护教育部重点实验室/经济林育种与栽培国家林业局重点实验室,长沙,410004;3. 湖北省农业科学院果树茶叶研究所,武汉,430064 |
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| 基金项目： | 国家自然科学基金项目（面上项目）（31272124）;国家林业局林业科技成果推广项目（[2014]53） |
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| 摘 要： | 梨品种S基因型鉴定对梨栽培中授粉品种选择和遗传育种都具有重要意义。本研究利用梨S-RNase基因荧光标记的特异引物PCR扩增获得梨品种荧光标记的cDNA特异产物;进一步完善梨S-RNase基因cDNA芯片,以被检测梨品种cDNA特异序列与梨S-RNase基因cDNA芯片杂交检测不同梨品种S基因型,并发现新的S-RNase基因。结果表明:利用梨S-RNase基因cDNA芯片鉴定了泸定王皮梨、兴山24号、弥渡百合等35个未知S基因型梨品种,确定了各品种的S基因型。结合PCRRFLP及DNA克隆和测序等技术,发现了7个新的S-RNase基因资源,获得了新S-RNase基因序列。序列分析表明各新S-RNase基因均具有S-RNase基因特异区域序列的典型特征;进化分析显示7个新S-RNase基因主要属于蔷薇科苹果亚科S-RNase类群,且存在种间和属间比种内和属内进化关系更近的现象。7个新的S基因分别命名为:PpS_(53)(Pyrus pyrifolia S53)、PpS_(54)、PpS_(55)、PpS_(56)、PpS_(57)、PpS_(58)和PpS_(59),GenBank登录号分别为:KX581753、KX581754、KX581755、KX581756、KX581757、KX581751和KX581752。
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| 关 键 词： | 梨 S-RNase基因 cDNA芯片 S基因型 进化分析 |
| 收稿时间： | 2016/8/17 0:00:00 |
| 修稿时间： | 2017/1/2 0:00:00 |
Detection of pear S-genotypes and evolutionary analysis of novel S-RNase genes identified by cDNA microarray-based method |
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| jiangnan,zhanglin,and.Detection of pear S-genotypes and evolutionary analysis of novel S-RNase genes identified by cDNA microarray-based method[J].Journal of Plant Genetic Resources,2017,18(3):520-529. |
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| Authors: | jiangnan zhanglin and |
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| Abstract: | Identification of S-genotypes of pear cultivars is useful for allocating suitable pollination trees and pear breeding program.The fluorescently-labeled specific cDNA sequences of S-RNases were obtained by PCR amplification using Cy3-labeled specific primers.The S-RNase cDNA microarray was further improved with which the pear S-genotype was detected and novel S-RNase genes were discovered.The S-genotypes of thirty-five pear cultivars,in total,including ‘ Luding Wangpili’,‘ Xingshan 24’ and‘ Midu Baihe’ etc,were identified and seven new Pyrus pyrifolia (N.L.Burman) Nakai.S-RNase genes were discovered.Then the DNA sequences of the seven new S-RNase genes were identified by DNA cloning and sequencing combined with PCR-RFLP method.Sequence analysis demonstrated that the seven new genes showed typical structural features with specific domains of S-RNase.Evolution analysis indicated that the seven genes belonged to the S-RNase group of Maloideae,Rosaceae,and the evolutionary relationship among S-RNases was closer between species or genera than intra-species or intra-genus.The seven S-RNases were named PpS53 (Pyruspyrifolia S53),PpS54,PpS55,PpS56,PpS57,PpS58 and PpS59,and had been deposited under the GenBank database with accession numbers KX581753,KX581754,KX581755,KX581756,KX581757,KX581751 and KX581752,respectively. |
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| Keywords: | S-RNase gene cDNA microarray S-genotype Evolution analysis |
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