Novel technologies in doubled haploid line development |
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| Authors: | Benjamin Trampe Xiaolong Tian Thomas Lübberstedt Shaojiang Chen |
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| Affiliation: | 1. Department of Agronomy, Iowa State University, Ames, IA, USA;2. National Maize Improvement Center of China, China Agricultural University, Beijing, China |
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| Abstract: | haploid inducer line can be transferred (DH) technology can not only shorten the breeding process but also increase genetic gain. Haploid induction and subsequent genome doubling are the two main steps required for DH technology. Haploids have been generated through the culture of immature male and female gametophytes, and through inter‐ and intraspecific via chromosome elimination. Here, we focus on haploidization via chromosome elimination, especially the recent advances in centromere‐mediated haploidization. Once haploids have been induced, genome doubling is needed to produce DH lines. This study has proposed a new strategy to improve haploid genome doubling by combing haploids and minichromosome technology. With the progress in haploid induction and genome doubling methods, DH technology can facilitate reverse breeding, cytoplasmic male sterile (CMS) line production, gene stacking and a variety of other genetic analysis. |
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| Keywords: | doubled haploid haploidization chromosome elimination genome doubling minichromosome |
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