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Distribution of Hydrogenase Genes in Desulfovibrio spp. and Their Use in Identification of Species from the Oil Field Environment
Authors:Gerrit Voordouw  Vincent Niviere  F Grant Ferris  Phillip M Fedorak  and Donald W S Westlake
Abstract:The distribution of genes for Fe], NiFe], and NiFeSe] hydrogenases was determined for 22 Desulfovibrio species. The genes for NiFe] hydrogenase were present in all species, whereas those for the Fe] and NiFeSe] hydrogenases had a more limited distribution. Sulfate-reducing bacteria from 16S rRNA groups other than the genus Desulfovibrio (R. Devereux, M. Delaney, F. Widdel, and D. A. Stahl, J. Bacteriol. 171:6689-6695, 1989) did not react with the NiFe] hydrogenase gene probe, which could be used to identify different Desulfovibrio species in oil field samples following growth on lactate-sulfate medium.
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