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接种微生物菌剂对猪粪堆肥过程中细菌群落多样性的影响
引用本文:解开治,徐培智,张发宝,陈建生,唐拴虎,黄旭,严超,顾文杰.接种微生物菌剂对猪粪堆肥过程中细菌群落多样性的影响[J].应用生态学报,2009,20(8):2012-2018.
作者姓名:解开治  徐培智  张发宝  陈建生  唐拴虎  黄旭  严超  顾文杰
作者单位:广东省农业科学院土壤肥料研究所广东省养分资源循环利用与耕地保育重点实验室,广州,510640
基金项目:国家科技支撑计划,广东省教育部产学研结合项目,广东省重大科技专项项目,广州市农业局招标项目
摘    要:利用PCR-DGGE方法研究了接种外源微生物菌剂对鲜猪粪高温好氧堆肥过程中细菌群落多样性的影响.结果表明:接种外源微生物菌剂可以促进堆肥的顺利进行,比不接种处理的高温期提前2 d.DGGE图谱分析表明,堆肥中优势细菌群落组成发生了明显的更迭现象,不同堆肥时期细菌群落的Shannon-Wiener指数呈显著差异.目的条带克隆测序结果表明,整个堆肥过程Clostridium stercorarium subsp. thermolacticum sp.一直是优势菌属,不经培养细菌、Bacillus coagulans sp.、Clostridium thermocellum sp.在接种外源微生物菌剂处理的第10、16天成为优势菌属,不经培养的Firmicutes sp.和不经培养的 delta proteobacterium分别在未接种外源微生物菌剂处理堆肥发酵的第5天和第16天成为优势菌属.非优势菌属Ureibacillus thermosphaericus、不经培养的Silvimonas sp.出现在堆肥腐熟后期,不经培养的土壤细菌主要出现在堆肥初期和高温初期.UPGMC聚类分析表明,接种外源微生物菌剂明显影响了堆肥不同时期的细菌群落结构组成.堆肥化过程中细菌DGGE图谱主成分分析表明,细菌群落变化主要受外源接种微生物菌剂的影响.

关 键 词:微生物菌剂  猪粪  好氧堆肥  细菌  群落多样性  变性梯度凝胶电泳(DGGE)
收稿时间:2008-12-09

Effects of microbial agent inoculation on bacterial community diversity in the process of pig manure composting
XIE Kai-zhi,XU Pei-zhi,ZHANG Fa-bao,CHEN Jian-sheng,TANG Shuan-hu,HUANG Xu,YAN Chao,GU Wen-jie.Effects of microbial agent inoculation on bacterial community diversity in the process of pig manure composting[J].Chinese Journal of Applied Ecology,2009,20(8):2012-2018.
Authors:XIE Kai-zhi  XU Pei-zhi  ZHANG Fa-bao  CHEN Jian-sheng  TANG Shuan-hu  HUANG Xu  YAN Chao  GU Wen-jie
Affiliation:Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation, Institute of Soil and Fertilizer, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
Abstract:PCR-DGGE method was adopted to study the effects of inoculating exogenous microbial agent on the bacterial community diversity in the process of fresh pig manure high-temperature aerobic composting. Exogenous microbial agent inoculation promoted the composting process, with the high-temperature period being advanced by 2 days than that of non-inoculation. DGGE pattern analysis showed that during composting, the dominant bacteria changed significantly, with the Shannon-Wiener index of bacterial community at different composting periods varied obviously. The sequencing of DGGE-distinguished bands showed that Clostridium stercorarium subsp. thermolacticum sp. was the dominant group in the whole composting process. Uncultured bacteria Bacillus coagulans sp. and Clostridium thermocellum sp. became the main groups on the 10th and 16th day after microbial agent inoculation, while uncultured Firmicutes sp. and delta proteobacterium became the dominant groups on the 5th and 16th days in the treatment non-inoculation, respectively. Un-dominant group Ureibacillus thermosphaericu sp. and uncultured  Silvimonas sp. appeared in the late period of well rotted composting, while uncultured soil bacteria mainly appeared in the initial and high-temperature periods. UPGMC cluster analysis showed that exogenous microbial agent inoculation obviously affected the bacterial community structure in different composting periods, and the main component analysis of DGGE patterns in composting process showed that the bacterial community was mainly affected by the exogenous microbial agent inoculation.
Keywords:microbial agent  pig manure  aerobic composting  bacteria  community diversity  denaturing gradient gel electrophoresis (DGGE)
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