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环境因子对乌桕内生真菌生长及脂肪酸的影响
引用本文:戴传超,余伯阳,徐增莱,袁生.环境因子对乌桕内生真菌生长及脂肪酸的影响[J].应用生态学报,2003,14(9):1525-1528.
作者姓名:戴传超  余伯阳  徐增莱  袁生
作者单位:1. 中国药科大学,南京,210009;南京师范大学生命科学学院,南京,210097
2. 中国药科大学,南京,210009
3. 南京师范大学生命科学学院,南京,210097
基金项目:中国科学院红壤生态开放试验站资助项目(2000-K-02)
摘    要:为探讨内生菌和植物的生态关系,以生物量和脂肪酸组分作为主要指标,研究了乌桕韧皮部分离获得的5种内生真菌(丝核菌、小菌核菌、小单头孢、毛壳菌、拟盘多毛孢)在不同环境因子下的生长.与合成培养基相比,在液体马铃薯培养基上发酵,生物量较高,脂肪酸不饱和指数较低;其脂肪酸主要为棕榈酸、油酸、亚油酸.与未添加乌桕汁的合成培养基相比,添加乌桕浸汁对小菌核菌生长有促进作用,对其余4种菌有抑制作用;脂肪酸不饱和指数均进一步增加.在合成培养基中添加NaCl培养小菌核菌,生物量均无显著差异;在0~0.5mol·L-1 NaCl时,脂肪酸不饱和指数无显著差异;在0.6~1.0mol·L-1 NaCl时随着盐浓度增加,脂肪酸不饱和指数却下降;表明该菌有较强的耐盐能力.添加植物油对小菌核菌菌丝生长有促进作用,其中在添加1.5%的植物油时,生物量最大;其脂肪酸随添加植物油而改变.以上特点均和二者的共生关系有关.

关 键 词:乌桕  内生真菌  菌丝生长  脂肪酸组分  环境因子
文章编号:1001-9332(2003)09-1525-04
修稿时间:2001年9月21日

Effect of environmental factors on the growth and fatty acid composition of five endophytic fungi from Sapium sebiferum
DAI Chuanchao,YU Boyang,XU Zenglai,YUAN Sheng.Effect of environmental factors on the growth and fatty acid composition of five endophytic fungi from Sapium sebiferum[J].Chinese Journal of Applied Ecology,2003,14(9):1525-1528.
Authors:DAI Chuanchao  YU Boyang  XU Zenglai  YUAN Sheng
Affiliation:China Pharmaceutical University, Life Science College of Nanjing Normal University, Nanjing, China. Dai-Chuan-Chao@sina.com
Abstract:Five fungi species including Rhizoctonia sp. , Sclerotium sp., Acremoniell sp., Chaetomium sp. and Pestalo-tiopsis sp. screened from the inner bark of Sapium sebiferum (Level) Roxb. were used to study their growth under different environmental factors, with the biomass and fatty acid composition as main indexes to elucidate the relationship between plant and its endophytic fungi. Compared with that from synthesized medium, a higher biomass was harvested when the fungi were cultured on liquid potato dextrose medium; the unsaturation index of fatty acid (IUFA) was lower, and the main fatty acids in the fungi were linoleic acid, palmitic acid and oleic acid. Compared with the fungi grown on the synthesized medium without Sapium sebiferum' s extract, the Sclerotium sp. growth was promoted, and the others were inhibited when the extract was added to the synthesized medium. At the same time, the IUFA were increased. When 0~1.0 mol.L-1 NaCl was added in Sclerotium sp. 's culture medium, the biomass was not changed notably; IUFA in 0 -0.5 mol.L-1 NaCl was not significantly different, while in the range of 0.6~1.0 mol.L-1 NaCl, the IUFA was decreased with increasing concentration of NaCl. It indicated that fungus had a stronger tolerance to salt. Sclerotium sp.'s growth was promoted, and the IUFA was increased when plant oil was added in the synthesized medium. The biomass was the highest by adding 1.5% plant oil. The results mentioned above were some way related to the symbiotic relations between the endophytic fungi and their host plant.
Keywords:Sapium sebiferum  Endophytic fungus  Mycelium growth  Fatty acid composition  Environmental factors  
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