| 镜鲤繁殖群体的遗传结构及微卫星标记与经济性状的相关性分析 |
| |
| 引用本文： | 孙新,魏振邦,孙效文,张研,鲁翠云.镜鲤繁殖群体的遗传结构及微卫星标记与经济性状的相关性分析[J].遗传,2008,30(3):359-366. |
| |
| 作者姓名： | 孙新 魏振邦 孙效文 张研 鲁翠云 |
| |
| 作者单位： | 1. 中国水产科学研究院黑龙江水产研究所, 哈尔滨 150070;2. 大连水产学院生命科学与技术学院, 大连 116023;3. 上海水产大学生命科学与技术学院, 上海 200090 ; |
| |
| 基金项目： | 国家重点基础研究发展计划(973计划)，国家公益性计划 |
| |
| 摘 要： | 选用35个多态性微卫星分子标记对天津换新良种场镜鲤一个繁殖群体的有效等位基因数(Ae)、观测杂合度(Ho)、期望杂合度(He)、多态信息含量(PIC) 等进行了检测, 以卡方检验估计群体Hardy-Weinberg平衡。结果表明：在35个基因座共检测到118个等位基因, 平均等位基因数为3.37个, 每个座位检测到的等位基因数2~7个不等, 平均有效等位基因数为2.16, 观测杂合度平均值0.431, 无偏期望杂合度的平均值为0.4736, 平均多态信息含量0.42, 说明这个群体属于中度多态, 遗传多样性水平不高。卡方检验的P值显示多于半数的位点都发生了偏离。并将35个基因座的不同基因型与个体的体重、体长值进行了连锁分析, 得到了4个与体重、体长连锁的基因型, 并将所得结果与鲤鱼体长性状QTL定位结果进行对比, 其中HLJ319标记与QTL定位结果基本一致。分析了几个严重偏离平衡的基因型, 并讨论出现这种现象的可能原因。
|
| 关 键 词： | 镜鲤 遗传结构 微卫星 相关分析 |
| 收稿时间： | 2007-09-18 |
| 修稿时间： | 2007年9月18日 |
Analysis of genetic structure of mirror carp population and correlation of microsatellite markers and economic traits |
| |
| SUN Xin,WEI Zhen-Bang,SUN Xiao-Wen,ZHANG Yan,LU Cui-Yun.Analysis of genetic structure of mirror carp population and correlation of microsatellite markers and economic traits[J].Hereditas,2008,30(3):359-366. |
| |
| Authors: | SUN Xin WEI Zhen-Bang SUN Xiao-Wen ZHANG Yan LU Cui-Yun |
| |
| Affiliation: | 1. Heilongjiang Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Harbin 150070, China;2. College of life Science and Technology, DaLian Fisheries University, Dalian 116023, China;3. College of Aqua-life Science and Technology, Shanghai Fisheries University, Shanghai 200090, China ; |
| |
| Abstract: | The genetic structure of one breeding population of mirror carp(Cyprinus carpio L.) was analysed using thirty-five polymorphic microsatellite markers. The effective number of alleles (Ae), observed heterozygosity (Ho), expected heterozygosity (He) and polymorphism information content(PIC) were all determined. The Hardy-Weinberg equilibrium was checked by chi-square test. For each locus, 2-7 alleles were detected, with a total of 118 alleles for 35 loci . The value of Ae, Ho, He and PIC showed that the genetic variation of the population was not high .The average effective number of alleles and the mean polymorphism information content were 2.16 and 0.42, respectively, and the observed and expected heterozygosity were 0.431 and 0.4736 , respectively.The probability value of chi-square test showed that more than half of the thirty-five loci have significantly (Plt;0.01) deviated from Hardy-Weinberg equilibrium. The correlation of genotype of each locus and individual phenotype data was analysed , and 4 loci seemed associated with body weight and body size . When compared with the result of QTL mapping of common carp, HLJ319 locus was consistent with the mapping result approximately, which was significantly correlated with body size . Several significantly deviated loci were also analysed, and the possible cause of the kind of deviation was discussed . |
| |
| Keywords: | |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《遗传》浏览原始摘要信息 |
|
点击此处可从《遗传》下载全文 |
|
