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大豆转基因体系的研究进展
引用本文:王萍,王罡,季静,吴颖.大豆转基因体系的研究进展[J].遗传,2004,26(6):969-976.
作者姓名:王萍  王罡  季静  吴颖
作者单位:解放军军需大学植物基因工程研究中心植物分子生物学研究室 长春 130062
基金项目:国家转基因植物研究和产业化专项基金
摘    要:从大豆的转基因方法和受体系统两个方面概述大豆转基因体系的最新研究进展,并讨论了大豆遗传转化的主要障碍及可能的解决途径。作者认为,以根癌农杆菌介导大豆子叶节和基因枪轰击大豆未成熟子叶是较有效的大豆遗传化系统。目前,在大豆遗传转化研究中存在着大豆组织培养体系有待于进一步完善、遗传转化率较低、重复性差、大豆受体基因型单一等问题,建立新的、高效和稳定的大豆组织培养体系,提高生产上栽培大豆品种的组织培养能力,改遗传转化现有的单一基因为多基因,可能是解决上述问题的有效途径。

关 键 词:受体系统  大豆  转基因方法  
文章编号:0253-9772(2004)06-0969-08
修稿时间:2003年6月20日

Research Progress on System of Transgene in Soybean
WANG Ping.Research Progress on System of Transgene in Soybean[J].Hereditas,2004,26(6):969-976.
Authors:WANG Ping
Affiliation:Laboratory of Plant Molecular Biology, Research Center For Plant Genetic Engineering, The Changchun University of Agriculture and Animal Sciences, Changchun 130062 China
Abstract:This review introduced the recent research progress on transgenic methods and system of receptor in soybean. The major obstacles of genetic transformation in soybean and possible approach for solving the problem were (also) discussed. Cotyledonary node via Agrobacterium tumefaciens-mediated and immature cotyledon via particle bombardment were thought to be the efficient systems of genetic transformation. Three problems existed in genetic transformation of soybean. The first one was that the system of tissue culture needs to be further improved. The second was that efficiency of genetic transformation was still low and difficult to be repeated. The last one was that restricted genotypes of soybean have been transformed successfully as a receptor. The path of solving these problems need to set a new and high efficient system for tissue culture in soybean. Also the number of target gene to be transformed will be increased from single gene to several genes at same time.
Keywords:Soybean  transgenic method  system of receptor
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