| IGFBP-3基因多态性及其与中国美利奴羊部分羊毛性状的关联性分析 |
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| 引用本文： | 沈敏,王文君,杨永林,甘尚权,何其宏,张永胜,王建华,马春萍,刘正山,刘守仁,李宁.IGFBP-3基因多态性及其与中国美利奴羊部分羊毛性状的关联性分析[J].遗传,2008,30(9):1182-1186. |
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| 作者姓名： | 沈敏 王文君 杨永林 甘尚权 何其宏 张永胜 王建华 马春萍 刘正山 刘守仁 李宁 |
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| 作者单位： | 1. 石河子大学动物科技学院, 石河子 832000;;2. 新疆兵团绵羊繁育生物技术重点实验室, 石河子 832000;;3. 江西农业大学食品科学与工程学院, 南昌 330045;;4. 新疆紫泥泉绵羊育种中心, 石河子 832025;;5. 中国农业大学农业生物技术国家重点实验室, 北京 100193 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 采用PCR-SSCP方法对中国美利奴羊和哈萨克羊中IGFBP-3基因的多态性进行了检测, 并对不同基因型与中国美利奴羊部分羊毛性状间的关联性进行了分析。结果在位于内含子1区的一段178 bp的扩增产物经SSCP分析后出现了3种基因型, 基因型AA、AB和BB及等位基因A、B在中国美利奴羊中的频率分别为0.70、0.24、0.06和0.82、0.18; 在哈萨克羊中的频率分别为0.87、0.13、0.00和0.93、0.07。序列分析发现: 在该序列的122位碱基表现多态性 (g.122 G>T)。所研究的两个群体在该位点上均处于Hardy-Weinberg不平衡状态(P<0.01)。不同基因型对部分羊毛性状有一定的影响: 不同基因型个体在剪毛后体重和净毛率上没有明显差异。AA、AB及BB基因型个体的羊毛伸直长度逐渐变短, 其中AA与AB基因型之间差异极显著(P<0.01)。AA型个体的剪毛量和羊毛密度要明显低于AB型(P<0.01)和BB型个体(P<0.05); 羊毛纤维直径则明显高于AB型(P<0.01)和BB型(P<0.05)个体。
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| 关 键 词： | 多态性 羊毛性状 IGFBP-3基因 中国美利奴羊 |
| 收稿时间： | 2007-12-25 |
| 修稿时间： | 2008-01-31 |
A novel polymorphism of IGFBP-3 gene and its relation-ship with several wool traits in Chinese Merino sheep |
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| SHEN Min,WANG Wen-Jun,YANG Yong-Lin,GAN Shang-Quan,HE Qi-Hong,ZHANG Yong-Sheng,WANG Jian-Hua,MA Chun-Ping,LIU Zheng-Shan,LIU Shou-Ren,LI Ning.A novel polymorphism of IGFBP-3 gene and its relation-ship with several wool traits in Chinese Merino sheep[J].Hereditas,2008,30(9):1182-1186. |
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| Authors: | SHEN Min WANG Wen-Jun YANG Yong-Lin GAN Shang-Quan HE Qi-Hong ZHANG Yong-Sheng WANG Jian-Hua MA Chun-Ping LIU Zheng-Shan LIU Shou-Ren LI Ning |
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| Affiliation: | 1. Animal Science and Technology college, Shihezi University, Shihezi 832000, China;2. Key Laboratory for Biotechnology of Sheep Breeding of Xinjiang Production & Construction Corps, Shihezi 832000, China;3. College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China;4. Ziniquan Research Institute of Sheep Breeding, Shihezi 832025, China;5. State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China ; |
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| Abstract: | In this study, PCR-SSCP analysis was used to identify genetic variation in IGFBP-3 gene in Chinese Merino and Kazakh sheep. A PCR product of 178 bp corresponding to partial intron1 illustrated three unique binding patterns by SSCP analysis. Frequencies of the genotype AA, AB, BB and allele A, B in Chinese Merino sheep were 0.70, 0.24, 0.06, and 0.82, 0.18 respectively , and they were 0.87, 0.13, 0.00, and 0.93, 0.07 respectively in Kazaka sheep. Sequence analysis revealed a G/T transversion at position 122 of the fragment. This polymorphic locus of IGFBP-3 gene was at Hardy-Weinberg dis-equilibrium (Plt;0.01) in the two breeds. Different genotypes slightly affected several wool traits of Chinese Merino sheep. The individuals of genotype AA, AB, and BB had no significant difference in post-shearing weight and clean wool rate. Sta-ple length (SL) was decreased with the genotype of AA, AB, and BB, and the difference between AA and AB was significant (Plt;0.01). Greasy fleece weight (GFW) and follicle density in individuals of genotype AA was significantly lower than that in individuals of genotype AB (Plt;0.01) and BB (Plt;0.05); Average fiber diameter (AFD) in individuals of genotype AA was significantly higher than that in individuals of genotype AB (Plt;0.01) and BB (Plt;0.05). |
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