| 11个绵羊品种MSTN基因非翻译区的变异 |
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| 引用本文： | 孟详人,郭军,赵倩君,马月辉,关伟军,刘娣,狄冉,乔海云,那日苏.11个绵羊品种MSTN基因非翻译区的变异[J].遗传,2008,30(12):1585-1590. |
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| 作者姓名： | 孟详人 郭军 赵倩君 马月辉 关伟军 刘娣 狄冉 乔海云 那日苏 |
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| 作者单位： | 1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;2. 黑龙江省农业科学院畜牧研究所, 哈尔滨 150086;3. 河北工程大学农学院, 邯郸 056000 ; |
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| 基金项目： | 国家科技支撑计划，国家自然科学基金，国家高技术研究发展计划(863计划) |
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| 摘 要： | 利用PCR-RFLP技术对特克塞尔羊、夏洛莱羊、小尾寒羊、蒙古羊、乌珠穆沁羊、阿勒泰羊、呼伦贝尔羊、塔什库尔干羊、多浪羊、湖羊和岗巴羊11个品种的345个个体的肌肉生长抑制素(Myostatin, MSTN)基因非翻译区(UTR)的变异进行了多态性分析。结果表明大小为271 bp和1 003 bp的扩增片段经限制性内切酶MboⅡ和BsaⅠ酶切后表现多态, 经卡方检验所有品种在该基因座位均处于平衡状态(P>0.05), 3种基因型在11个绵羊品种中的分布差异极显著(P<0.01)。通过限制性内切酶HpyCH4Ⅳ 酶切实验, 证明我国9个地方绵羊品种不存在特克塞尔绵羊中发现的导致肌肉发达的SNP位点, 并在3′UTR区发现了个别碱基突变位点能够形成miRNA作用的靶基序, 测序表明3′UTR区的突变频率较高。
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| 关 键 词： | MSTN UTR PCR-RFLP MicroRNA 多态性 |
| 收稿时间： | 2008-03-01 |
| 修稿时间： | 2008-09-12 |
Variation of MSTN gene UTR in eleven sheep breeds |
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| MENG Xiang-Ren,GUO Jun,ZHAO Qian-Jun,MA Yue-Hui,GUAN Wei-Jun,LIU Di,DI Ran,QIAO Hai-Yun,NA Ri-Su.Variation of MSTN gene UTR in eleven sheep breeds[J].Hereditas,2008,30(12):1585-1590. |
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| Authors: | MENG Xiang-Ren GUO Jun ZHAO Qian-Jun MA Yue-Hui GUAN Wei-Jun LIU Di DI Ran QIAO Hai-Yun NA Ri-Su |
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| Affiliation: | 1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2. Institute of Animal Science, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China;3. College of Agriculture, Hebei University of Engineering, Handan 056000, China ; |
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| Abstract: | PCR-RFLP was applied to analyze the polymorphism of MSTN gene UTR in 345 sheep that comprised of eleven sheep breeds, namely Texel sheep, Charolais sheep, Small-tailed Han sheep, Monggolian sheep, Ujumqin sheep, Altay Fat-rumped sheep, Hulunbeir sheep, Tashikurgan sheep, Duolang sheep, Hu sheep, and Gangba sheep. A 271 bp and a 1 003 bp long PCR products were digested with MboⅡand BsaⅠto demonstrate polymorphism in the eleven sheep breeds, which were all at Hardy-Weinberg equilibrium (P>0.05). The distribution of 3 genotypes in 11 sheep breeds was significantly different (P<0.01). Digestion of the PCR products with HpyCH4Ⅳ proved that 9 domestic local sheep breeds were different from Texel sheep in the SNP site that was associated with muscularity. The individual mutation base could gener-ate the motifs for miRNA in the 3′UTR, and sequencing analysis demonstrated high frequency of mutation in the 3′UTR region. |
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| Keywords: | MSTN UTR PCR-RFLP MicroRNA |
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