| 水稻ILP标记遗传图谱的构建 |
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| 引用本文： | 赵向前,吴为人.水稻ILP标记遗传图谱的构建[J].遗传,2008,30(2):225-230. |
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| 作者姓名： | 赵向前 吴为人 |
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| 作者单位： | 1. 浙江大学农业与生物技术学院, 杭州 310029;2. 浙江省嘉兴市农业科学研究院, 嘉兴 314016 ; |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 内含子长度多态性(ILP)是一种基于PCR的新型分子标记, 具有许多突出的优点。我们先前利用已公布的籼稻品种93-11和粳稻品种日本晴的基因组序列数据, 已开发了172个水稻ILP标记。为了检验这些ILP标记的可靠性及其在遗传作图中的可用性, 利用一个BC1F1(日本晴/93-11//日本晴)群体, 构建了一张含172个ILP标记座位和13个SSR标记座位的水稻遗传图谱, 总长度为1 905.7 cM。比较显示, 图谱上所有标记的顺序与其物理顺序完全一致, 证明了利用ILP标记进行遗传作图的可行性和有效性。文中还对标记偏分离现象进行了分析, 发现在第6号染色体短臂上存在一个严重偏分离的区域。
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| 关 键 词： | 遗传图谱 ILP标记 水稻 |
| 收稿时间： | 2007-08-22 |
| 修稿时间： | 2007年8月22日 |
Construction of a genetic map based on ILP markers in rice |
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| ZHAO Xiang-Qian,WU Wei-Ren.Construction of a genetic map based on ILP markers in rice[J].Hereditas,2008,30(2):225-230. |
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| Authors: | ZHAO Xiang-Qian WU Wei-Ren |
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| Affiliation: | 1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; 2. Jiaxing Academy of Agricultural Science, Jiaxing 314016, China ; |
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| Abstract: | Intron length polymorphism (ILP) is a new type of PCR-based molecular markers with many advantages. We had previously developed 172 ILP markers in rice using the published genome sequence data of indica cultivar 93-11 and ja-ponica cultivar Nipponbare. In order to examine the reliability and the applicability of these ILP markers to genetic map-ping, we constructed a rice genetic map consisting of 172 ILP and 13 SSR markers with a total length of 1 905.7 cM using a BC1F1 (Nipponbare/93-11//Nipponbare) population. Comparison showed that the order of the markers in this map was ex-actly the same as their physical order, verifying the feasibility and efficiency of using ILP markers for genetic mapping. We also investigated the phenomenon of marker segregation distortion and found a region of serious segregation distortion in the short arm of chromosome 6. |
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