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| DArT技术的原理及其在植物遗传研究中的应用 | |
| 引用本文： | 洪义欢,肖宁,张超,苏琰,陈建民.DArT技术的原理及其在植物遗传研究中的应用[J].遗传,2009,31(4):359-364. |
| 作者姓名： | 洪义欢 肖宁 张超 苏琰 陈建民 |
| 作者单位： | 1. 扬州大学生物科学与技术学院,扬州,225009 2. 江苏里下河地区农业科学研究所,扬州,225000 |
| 摘 要： | DArT是一种依赖于基因芯片杂交基础之上的分子标记技术, 由于其具有省时、高通量、低花费、无需预知研究对象的DNA序列等优点而被应用到植物遗传研究中。文章介绍了DArT技术的原理、特点、具体操作方法及其目前在植物遗传研究中的应用。 |
| 关 键 词： | 分子标记 DArT技术 杂交 |
| 收稿时间： | 2008-10-04 |
| 修稿时间： | 2008-11-07 |
Principle of diversity arrays technology (DArT) and its applications in genetic research of plants |
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| HONG Yi-Huan,XIAO Ning,ZHANG Chao,SU Yan,CHEN Jian-Min.Principle of diversity arrays technology (DArT) and its applications in genetic research of plants[J].Hereditas,2009,31(4):359-364. | |
| Authors: | HONG Yi-Huan XIAO Ning ZHANG Chao SU Yan CHEN Jian-Min |
| Affiliation: | 1. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China; 2. Agricultural Scicece Institute of Jiangsu Lixiahe District, Yangzhou 225000, China |
| Abstract: | Diversity arrays technology (DArT) is a microarray hybridization-based molecular marker technique, and a novel method to discover genetic polymorphic markers. DArT has recently been applied in genetic research of plants be-cause of its characteristics of sequence-independent, high–throughput, fast, low-cost. We briefly introduce the principle, characteristics, protocol of DArT and its application in genetic research of plants. |
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