首页 | 官方网站   微博 | 高级检索  
     

利用基因芯片检测转基因作物
引用本文:黄迎春,孙春昀,冯红,胡晓东,尹海滨.利用基因芯片检测转基因作物[J].遗传,2003,25(3):307-310.
作者姓名:黄迎春  孙春昀  冯红  胡晓东  尹海滨
作者单位:成都百奥生物信息科技有限公司,成都东胜街8号 610015 Chengdu Bioinformation Technique Incorporation,Dongsheng Rood No.8,Chengdu 610015,China
摘    要:选用常用的两种报告基因、两种抗性基因、两种启动子序列和两种终止子序列为探针,将其PCR扩增产物用MicroGrid Ⅱ型全自动点样仪按矩阵排列点样于包埋有氨基的载玻片上,制备成转基因作物检测型基因芯片。利用该芯片对4种转基因水稻、木瓜、大豆、玉米进行检测,结果表明,该芯片能对转基因作物做出快速、准确的检测。 Abstract:Some selected available sequences of reporter genes,resistant genes,promoters and terminators are amplified by PCR for the probes of transgenic crop detection gene chip.These probes are arrayed at definite density and printed on the surface of amino-slides by bioRobot MicroGrid Ⅱ.Results showed that gene chip worked quickly and correctly,when transgenic rice,pawpaw,maize and soybean were applied.

关 键 词:transgenic  crop  检测  Key  words  gene  chip  转基因作物  基因芯片  
文章编号:0253-9772(2003)03-0307-04
修稿时间:2002年5月17日

Detection of Transgenic Crop with Gene Chip
Ying-Chun Huang,Chun-Yun Sun,Hong Feng,Xiao-Dong Hu,Hai-Bin Yin.Detection of Transgenic Crop with Gene Chip[J].Hereditas,2003,25(3):307-310.
Authors:Ying-Chun Huang  Chun-Yun Sun  Hong Feng  Xiao-Dong Hu  Hai-Bin Yin
Affiliation:Chengdu Bioinformation Technique Incorporation, Chengdu 610015, China. ychuang72@yahoo.com.cn
Abstract:Some selected available sequences of reporter genes,resistant genes, promoters and terminators are amplified by PCR for the probes of transgenic crop detection gene chip. These probes are arrayed at definite density and printed on the surface of amino-slides by bioRobot MicroGrid II. Results showed that gene chip worked quickly and correctly, when transgenic rice, pawpaw,maize and soybean were applied.
Keywords:gene chip  transgenic crop  detection
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《遗传》浏览原始摘要信息
点击此处可从《遗传》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司    京ICP备09084417号-23

京公网安备 11010802026262号