| 玉米抗甘蔗花叶病毒基因的比较定位 |
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| 引用本文： | 吕香玲,李新海,谢传晓,郝转芳,吉海莲,史利玉,张世煌.玉米抗甘蔗花叶病毒基因的比较定位[J].遗传,2008,30(1):101-108. |
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| 作者姓名： | 吕香玲 李新海 谢传晓 郝转芳 吉海莲 史利玉 张世煌 |
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| 作者单位： | 1. 中国农业科学院作物科学研究所, 农作物基因资源与基因改良国家重大科学工程/农业部作物遗传育种重点开放实验室, 北京 100081; 2. 沈阳农业大学农学院, 沈阳 110161 ; |
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| 基金项目： | 国家引进国际先进农业科学技术计划(948计划),国家高技术研究发展计划(863计划) |
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| 摘 要： | 收集了玉米抗甘蔗花叶病毒基因/QTL定位信息, 借助玉米遗传图谱IBM2 2005 Neighbors进行了整合。在国内外研究中, 累计报道81个抗病毒基因位点, 分布在玉米7条染色体上, 比较定位发现这些位点集中分布于第3和6染色体。采用元分析技术, 确定3个“一致性”抗病毒QTL, 其中1个位于第3染色体, 在遗传图谱IBM2 2005 Neighbors上覆盖的范围为6.44 cM; 2个位于第6染色体, 覆盖范围分别为6.16 cM和27.48 cM。借助比较基因组学策略, 在第3染色体“一致性”QTL区间内筛选出4个抗病位置候选基因。该研究结果为确定和克隆抗病主效基因提供了基础。
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| 关 键 词： | 玉米 甘蔗花叶病毒 数量性状位点 比较定位 |
| 收稿时间： | 2007-07-19 |
| 修稿时间： | 2007年7月19日 |
Comparative QTL mapping of resistance to sugarcane mosaic virus in maize based on bioinformatics |
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| LV Xiang-Ling,LI Xin-Hai,XIE Chuan-Xiao,HAO Zhuan-Fang,JI Hai-Lian,SHI Li-Yu,ZHANG Shi-Huang.Comparative QTL mapping of resistance to sugarcane mosaic virus in maize based on bioinformatics[J].Hereditas,2008,30(1):101-108. |
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| Authors: | LV Xiang-Ling LI Xin-Hai XIE Chuan-Xiao HAO Zhuan-Fang JI Hai-Lian SHI Li-Yu ZHANG Shi-Huang |
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| Affiliation: | 1. Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement/Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Beijing 100081, China; 2. College of Agronomy, Shenyang Agricultural University, Shenyang 110161, China ; |
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| Abstract: | The development of genomics and bioinformatics offers new tools for comparative gene mapping. In this paper, an integrated QTL map for Sugarcane mosaic virus (SCMV) resistance in maize was constructed by compiling a total of 81 QTL loci available with the Genetic Map IBM2 2005 Neighbors as reference. These 81 QTL loci were scattered on 7 chromosomes of maize, and most of them was clustered on chromosome 3 and 6. By using meta- analysis method, we identified one and two "consensus QTLs" on chromosomes 3 and 6, respectively. These three QTLs cover the genetic distances of 6.44 cM, 6.16 cM and 27.48 cM on the genetic map IBM2 2005 Neighbors, respectively. Four positional candidate resistant genes were identified within the "consensus QTL" on chromosome 3 via comparative genomics strategy. These results suggested that application of the combined meta-analysis within a species with sequence homologous comparison in a related model plant is an efficient approach to identify the major QTL and its candidate gene(s) for the target traits. The results of this study provided useful information for identifying and cloning of the major gene(s) conferring resistance to SCMV in maize. |
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