Conditional UAS-targeted repression in Drosophila |
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| Authors: | Pascual Alberto Huang Kai-Lian Preat Thomas |
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| Affiliation: | Développement, Evolution, Plascticité du Système Nerveux, C.N.R.S. 1 Avenue de la Terrasse, 91190 Gif-sur-Yvette, France. |
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| Abstract: | The Gal4–UAS enhancer trap system is useful for driving gene expression in various tissues. A new tool that extends Gal4 technology is described here. A fusion protein containing the Gal4 binding domain and the repression domain of the isolator suppressor of hairy wing was placed under the control of a heat shock-inducible promoter. The construct mediates the conditional repression of genes located downstream of a UAS sequence. The repressive effects of the chimeric protein on fasII gene expression were tested by western-blot analysis and in brain sections of adult Drosophila. Owing to the increasing number of Gal4 and UAS transgenic lines, this versatile system will facilitate the study of gene function. |
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