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Site-directed cleavage of RNA.
Authors:S Shibahara  S Mukai  T Nishihara  H Inoue  E Ohtsuka  and H Morisawa
Abstract:Using complementary chimeric oligonucleotides containing deoxyribonucleotides and 2'-O-methylribonucleotides (1), enzymatically synthesized RNA (90 mer) were cleaved at a single site with Escherichia coli RNaseH, either at a hairpin loop or at a stem region. Especially, site-specific cleavage occurred in even the target region being enclosed within a stable, base-paired stem. The method is proved to be generally applicable to RNA containing secondary structures.
Keywords:
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