A new endonuclease recognizing the deoxynucleotide sequence CCNNGG from the cyanobacterium Synechocystis 6701. |
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| Authors: | F Callja N Tandeau de Marsac T Coursin H van Ormondt and A de Waard |
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| Affiliation: | F Calléja, N Tandeau de Marsac, T Coursin, H van Ormondt, and A de Waard |
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| Abstract: | A new sequence-specific endonuclease from the cyanobacterium Synechocystis species PCC 6701 has been purified and characterized. This enzyme, SecI, is unique in recognizing the nucleotide sequence: 5' -CCNNGG-3' 3' -GGNNCC-5' and cleaves it at the position indicated by the symbol. Two other restriction endonucleases, SecII and SecIII, found in this organism are isoschizomers of MspI and MstII, respectively. |
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