Ability of the endangered species Papaver fauriei to produce hybrids with a cultivated poppy (Papaver sp.) |
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| Authors: | Masumi Yamagishi Tetsuya Kondo Kazuki Kosugi |
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| Affiliation: | 1. Research Faculty of Agriculture, Hokkaido University, N9W9, Kita‐ku, Sapporo, 060‐8589, JapanThese authors contributed equally to the article.;2. Research Faculty of Agriculture, Hokkaido University, N9W9, Kita‐ku, Sapporo, 060‐8589, Japan;3. Informative Center for the Natural Environment of Rishiri, Rishiri, Hokkaido, 097‐0401, Japan |
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| Abstract: | Papaver fauriei is an endemic and endangered species that grows only on the gravelly alpine slopes of Mt. Rishiri, Japan. Cultivated poppy (Papaver sp.), the species name of which is unknown, has been introduced to the natural habitats of P. fauriei through human activities. Because the appearance and internal transcribed spacer (ITS) sequences of these two poppies are highly similar, it is of concern that they could produce hybrids in their natural habitats. Thus, first, the ability of these two poppies to produce hybrids was analyzed by artificial fertilization in this study. A large number of seeds were produced by reciprocal crosses between P. fauriei and the cultivated poppy, comparable with the number of seeds obtained by self‐ or cross‐fertilization of P. fauriei or the cultivated poppy. In addition, high germination was observed for seeds obtained from crosses between the two poppies, and deleterious phenotypes, such as albinism and dwarfism, were not detected in the F1 generation. These results indicate that after pollination, there is no reproductive isolation between the two poppies. Second, we sequenced the internal transcribed spacer (ITS) region of 240 poppy individuals collected from the gravelly alpine slopes of Mt. Rishiri, and 66 showed the sequence of P. fauriei, whereas 174 showed the sequence of the cultivated poppy. However, the ITS sequence that confirms hybridism between the two poppies was not detected in these individuals, indicating that hybridization of P. fauriei and the cultivated poppy rarely occurs under natural conditions. Unknown mechanism(s) appear to prevent cross‐pollination between the two poppies. |
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| Keywords: | artificial hybridization conservation of endangered plants hybrid incompatibility internal transcribed spacer special protection zone of Rishiri National Park |
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