| Hg2+胁迫对浮萍体细胞DNA一级结构和抗氧化酶系的损伤(英文) |
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| 引用本文： | 徐楠,施国新,曾晓敏,丁小余,徐勤松,陈源.Hg2+胁迫对浮萍体细胞DNA一级结构和抗氧化酶系的损伤(英文)[J].植物生态学报,2003,27(3):299-303. |
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| 作者姓名： | 徐楠 施国新 曾晓敏 丁小余 徐勤松 陈源 |
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| 作者单位： | 徐楠（南京师范大学生命科学学院,南京,210097）;施国新（南京师范大学生命科学学院,南京,210097）;曾晓敏（南京师范大学生命科学学院,南京,210097）;丁小余（南京师范大学生命科学学院,南京,210097）;徐勤松（南京师范大学生命科学学院,南京,210097）;陈源（南京师范大学生命科学学院,南京,210097） |
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| 基金项目： | This project was
supported by the National Natural Science Foundation of China (39770046), Key Program for
Science and Technology of Education Ministry of China (01043) and the Natural Science
Foundation of Provincial Educational Committee of Jiangsu, China |
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| 摘 要： | 主要从DNA一级结构及抗氧化酶系变化两方面，研究了Hg2+胁迫下浮萍体细胞的损伤。结果表明：运用随机扩增多态性DNA法（Random amplified polymorphism DNA， RAPD）和DNA梯法（DNA Ladder），5～10 mg·L-1 Hg2+处理组可检测到基因组DNA的明显损伤，20 mg·-1 Hg2+已导致细胞坏死；RAPD法较DNA Ladder法更灵敏。本文还发现，活性氧和抗氧化酶系很可能参与了浮萍体细胞凋亡过程。低浓度的Hg2+胁迫可刺激抗氧化酶活性升高，以清除体内活性氧，而一旦活性氧水平超出一定域值，抗氧化酶活性急速下降，导致细胞凋亡。
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| 关 键 词： | Hg2+浮萍 DNA损伤 抗氧化酶 RAPD D |
| 修稿时间： | 2002年7月29日 |
DAMAGE TO DNA PRIMARY STRUCTURE AND ANTIOXIDANT ENZYMES IN LEMNA MINOR
INDUCED BY HG2+ |
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| XU Nan,SHI Guo-Xin,ZENG Xiao-Min,DING Xiao-Yu,XU Qin-Song and CHEN Yuan.DAMAGE TO DNA PRIMARY STRUCTURE AND ANTIOXIDANT ENZYMES IN LEMNA MINOR
INDUCED BY HG2+[J].Acta Phytoecologica Sinica,2003,27(3):299-303. |
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| Authors: | XU Nan SHI Guo-Xin ZENG Xiao-Min DING Xiao-Yu XU Qin-Song and CHEN Yuan |
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| Abstract: | The damage toLemna minor cells induced by Hg2+ was studied in this article. Most damage occurred to DNA primary structure and changes in antioxidant enzyme activities were investigated by using RAPD and DNA ladder methods. The results showed that obvious damage to DNA was found in the process of apoptosis induced by 5-10 mg·L-1 Hg2+, and 20 mg·L-1 Hg2+ had already caused necrotic injury. The RAPD method was the more sensitive of the two methods, and so could be considered as an important method for monitoring apoptosis. The results also indicated that reactive oxygen species (ROS) and antioxidant enzymes are involved in the process of apoptosis. The activities of antioxidant enzymes could be stimulated to eliminate active oxygen by exposing the Lemna minor to low Hg2+ concentration; the cells declined rapidly when ROS were unable to be eliminated effectively. |
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| Keywords: | Hg2+ Lemna minor DNA damage Antioxidant enzymes RAPD DNA Ladder |
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