Functionality of insect‐cell‐derived colorectal cancer vaccine candidate protein EpCAM‐Fc in human dendritic cells |
| |
| Authors: | Yo Seph Park Jeong‐Hwan Lee Jin‐Yeon Park Han Sung Hwang Martin Zenke Dong Wook Han Young Sun Kang Kisung Ko Kinarm Ko |
| |
| Affiliation: | 1. Department of Stem Cell Biology, Konkuk University School of Medicine, Seoul, Korea;2. Center for Stem Cell Research, Institute of Advanced Biomedical Science, Konkuk University, Seoul, Korea;3. Department of Medicine, College of Medicine, Chung‐Ang University, Seoul, Korea;4. Department of Biomedical Science and Technology, Institute of Advanced Biomedical Science, Konkuk University, Seoul, Korea;5. Department of Obstetrics and Gynecology, Konkuk University School of Medicine, Seoul, Korea;6. Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, Aachen, Germany;7. Research Institute of Medical Science, Konkuk University, Seoul, Korea |
| |
| Abstract: | The baculovirus–insect cell expression system is widely used to produce recombinant proteins for various biomedical applications. Our previous study demonstrated that EpCAM, a colorectal cancer vaccine candidate protein, can be expressed in the baculovirus–insect cell expression system. However, its functionality (the ability to elicit an immune response), which is important for its possible use as a colorectal cancer vaccine for immunotherapy, still needed to be confirmed. In this study, we examined the ability of recombinant EpCAM to induce maturation of immature dendritic cells (DCs) derived from CD34+ cells isolated from human umbilical cord blood. We demonstrated that EpCAM induces the expression of four DC maturation markers: CD80, CD83, CD86 and MHC II. These results suggest that EpCAM produced in the baculovirus–insect cell expression system is functional in terms of its ability to trigger maturation of human DCs. |
| |
| Keywords: | baculovirus colorectal cancer dendritic cells EpCAM human umbilical cord blood insect cell expression system |
|
|
