Reduction of protein kinase C α (PKC‐α) promote apoptosis via down‐regulation of Dicer in bladder cancer |
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| Authors: | Zhenming Jiang Chuize Kong Zhe Zhang Yuyan Zhu Yuxi Zhang Xi Chen |
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| Affiliation: | 1. Department of Urology, The First Affiliated Hospital of China Medical University, Shenyang, China;2. Department of Pharmacology, The First Affiliated Hospital of China Medical University, Shenyang, China |
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| Abstract: | In clinic, we examined the expression of protein kinase C (PKC)‐α and Dicer in the samples of bladder cancer patients, and found that the two proteins have a line correlation. Our study confirmed this correlation existing by clearing the decreasing expression of Dicer after the PKC‐α knockdown. Treatment of bladder cancer cell lines (T24, 5637) with the PKC‐α or Dicer knockdown and the PKC inhibitors (Calphostin C and Gö 6976) can promote the apoptosis. Inhibition of PKC can increase the activities of caspase‐3 and PARP, however, decrease the expression of Dicer. And knockdown of the PKC‐α or Dicer can also activate the caspase‐3 or the PARP. Considering the reduction of PKC‐α can induce the Dicer down‐regulation, we make the conclusion that the reduction of PKC‐α can promote the apoptosis via the down‐regulation of Dicer in bladder cancer. |
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| Keywords: | PKC‐α Dicer apoptosis bladder cancer caspase‐3 |
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