| 分子生物学方法在微生物多样性研究中的应用 |
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| 引用本文： | 杨永华,姚健.分子生物学方法在微生物多样性研究中的应用[J].生物多样性,2000,8(3):337-342. |
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| 作者姓名： | 杨永华 姚健 |
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| 作者单位： | 南京大学生物科学与技术系,南京,210093 |
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| 基金项目： | 国家自然科学基金资助项目!(编号 :396 70 15 1) |
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| 摘 要： | 微生物多样性是生物多样性的重要组成部分。由于微生物和大生物（动、植物）相比，存在着多种显著差异，因此其多样性，保护及利用也有所不同，尤其是研究方法亟待完善，提高。近年来，分子生物学方法广泛用于微生物多样性的研究并取得了一系列研究成果。本文从四个方面加以介绍：１）微生物总ＤＮＡ制备及其遗传多样性检测方法；２）１６ＳｒＲＮＡ基因序列研究；３）核酸杂交分析技术；４）ＤＮＡ动力学的研究。今后的发展趋势是加
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| 关 键 词： | 微生物多样性 遗传多样性 核酸杂交 DNA动力学 |
| 收稿时间： | 1999-8-17 |
| 修稿时间： | 1999-08-17 |
Molecular techniques and their application to the study of microbial diversity |
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| YANG Yong-Hua,YAO Jian.Molecular techniques and their application to the study of microbial diversity[J].Biodiversity Science,2000,8(3):337-342. |
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| Authors: | YANG Yong-Hua YAO Jian |
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| Abstract: | Microbial diversity is an important part of biodiversity. Microbial diversity research and its conservation and utilization are quite different from those of macroorganisms including animals and plants. In particular, it is necessary to develop new techniques suitable for microbial diversity research. Recently, as the development of modern molecular biology, several molecular techniques have been used to study microbial diversity , including 1) total DNA extraction of microbe and its genetic diversity assay, 2) 16S rRNA gene sequence analysis, 3) nucleic acid hybridization, and 4) DNA kinetics. In order to promote extensive and intensive research on microbial diversity, it's necessary to integrate these methods to enhance a combination of traditional and modern techniques, and to develop novel method. |
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| Keywords: | microbial diversity DNA amplification genetic diversity 16S rRNA gene sequence nucleic acid hybridization DNA kinetics |
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