| 盐度对大麻哈鱼幼鱼存活率、鳃ATP酶活力及其组织结构的影响 |
| |
| 引用本文： | 李培伦,刘伟,王继隆,鲁万桥,崔康成,高文燕.盐度对大麻哈鱼幼鱼存活率、鳃ATP酶活力及其组织结构的影响[J].水生生物学报,2020,44(3):562-569. |
| |
| 作者姓名： | 李培伦 刘伟 王继隆 鲁万桥 崔康成 高文燕 |
| |
| 作者单位： | 中国水产科学研究院黑龙江水产研究所;上海海洋大学水产与生命学院 |
| |
| 基金项目： | 农业农村部财政专项物种资源保护项目(2010-2019);中央级公益性科研院所基本科研业务费专项(HSY201712Q)资助。 |
| |
| 摘 要： | 为研究不同盐度对大麻哈鱼幼鱼存活率、鳃ATP酶活力及其组织结构的影响,试验共设置4个盐度组(S0、S8、S16、S24),试验周期42d,解剖取鱼鳃测定ATP酶活力,并运用组织切片及扫描电镜技术观察其鳃组织结构的变化。结果显示:S8和S16组大麻哈鱼幼鱼存活率最高,均达到98.89%, S0组存活率为94.45%,而S24组存活率最低,为83.34%。随着盐度的升高,大麻哈鱼幼鱼鳃组织Na^+/K^+-ATP和Ca^2+/Mg^2+-ATP酶活力均呈现出先升高后下降的趋势且酶活力最高的均为S8组、最低的为S24组。鳃丝宽度随盐度升高逐渐增大且各组之间呈现出显著性差异(P<0.05),而鳃小片长度和宽度均随盐度升高逐渐减小。扫描电镜结果表明随着盐度的升高线粒体丰富细胞数量逐渐增多、顶膜变小且微绒毛消失;同S0组相比, S8组和S16组鳃丝表面扁平上皮细胞之间的轮廓更加清楚且环形微脊条纹清晰,而S24组鳃丝表面扁平上皮细胞之间界限模糊,环形微脊间有融合或间断情况;鳃小片底部扁平上皮细胞表层结构由清晰逐渐变得混乱、气孔数量逐渐减少且孔径变小。因此推测大麻哈鱼幼鱼在降海阶段适宜的盐度生存范围可能介于8‰-16‰,具体有待进一步研究。
|
| 关 键 词： | 大麻哈鱼 盐度 鳃丝 鳃小片 线粒体丰富细胞 |
| 收稿时间： | 2019-03-28 |
EFFECTS OF SALINITY ON SURVIVAL RATE,GILL ATPASE ACTIVITY AND GILL STRUCTURE OF JUVENILES CHUM SALMON |
| |
| Affiliation: | (Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Science,Harbin 150070,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China) |
| |
| Abstract: | In this study, chum salmon (Oncorhynchus keta) were cultured in different salinities for 0, 8, 16 and 24 for 42 days to investigate the effects of salinity level on survival rate, ATP enzyme activity and gill structure. Chum salmon gills were dissected to measure ATP enzyme activity and to observe the gill structure under different salinities using histological photomicrography and scanning electron microscopy technologies for determining the mechanism of catadromous migration. The results showed that the survival rates of the S8 and S16 groups were the highest, reaching 98.89%; the survival rate of the S0 group was 94.45%, and for the S24 group, it was 83.34%. The Na+/K+-ATP and Ca2+/Mg2+-ATP enzyme activities first increased and then decreased with increasing salinity, and the highest enzyme activity was found for the S8 group, and the lowest enzyme activity was found for the S24 group. The gill filament width increased with increasing salinity, while the gill lamella length and width decreased, and the differences between these groups were significant (P<0.05). The number of mitochondria-rich cells gradually increased, the apical membrane area decreased and the microvilli disappeared with the increase in salinity, as observed by electron microscopy. The boundaries between the flat epithelial cells on the surface of the gill filament and the circular micro ridges in the S8 group and the S16 group were clearer than those of the S0 group, while the boundaries between the flat epithelial cells on the surface of the gill filament were blurry, and fusion or discontinuity between annular microridges was observed in the S24 group. The surface structures of the flat epithelial cells at the bottom of the gill patches gradually changed from being organized to being disorganized, the number of stomata gradually decreased, and the pore sizes were reduced. According to these results, the suitable salinity range in which juvenile chum salmon survive was suggested to be between 8‰ and 16‰ during the catadromous migration stage, a supposition that needs to be confirmed by further study. |
| |
| Keywords: | Chum salmon Salinity Gill filament Gill lamella Mitochondria-rich cell |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《水生生物学报》浏览原始摘要信息 |
|
点击此处可从《水生生物学报》下载全文 |
|
