| 饲料中添加抗菌肽Surfactin对吉富罗非鱼肠道健康的影响 |
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| 引用本文： | 翟少伟,史庆超,陈学豪.饲料中添加抗菌肽Surfactin对吉富罗非鱼肠道健康的影响[J].水生生物学报,2016,40(4):823-829. |
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| 作者姓名： | 翟少伟 史庆超 陈学豪 |
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| 作者单位： | 集美大学水产学院,厦门,361021 |
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| 基金项目： | “十二五”农村领域国家科技计划课题“功能性肽产品的创制与应用”(2013BAD10B03)资助~~ |
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| 摘 要： | 为研究饲料中添加抗菌肽Surfactin对吉富罗非鱼(GIFT, Oreochromis niloticus)肠道健康指标的影响, 实验将平均体重为(12.010.03) g/尾的320尾吉富罗非鱼, 随机分为4组, 每组4个重复, 每个重复20尾鱼, 分别投喂抗菌肽Surfactin添加水平为0(对照组)、50、100和200 mg/kg的试验饲料7周。结果显示: 与对照组相比, 吉富罗非鱼饲料中添加抗菌肽Surfactin可使肠道皱襞高度显著增加(P0.05), 肌层厚度无显著变化(P0.05); 大肠杆菌数量显著降低(P0.05), 乳酸杆菌数量显著增加(P0.05), 细菌总数无显著变化(P0.05); 显著提高肠道总抗氧化能力水平、谷胱甘肽过氧化物酶活性和超氧化物歧化酶活性(200 mg/kg抗菌肽添加组除外)(P0.05), 显著降低肠道丙二醛水平(P0.05), 对过氧化氢酶活性无显著影响(P0.05); 吉富罗非鱼肠道健康指标以50 mg/kg抗菌肽Surfactin添加组最佳。试验表明, 吉富罗非鱼饲料中适量添加抗菌肽Surfactin可增加肠道皱襞高度、调节肠道菌群和提高肠道抗氧化能力而改善肠道健康状态。
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| 关 键 词： | 抗菌肽 吉富罗非鱼 肠道健康 |
| 收稿时间： | 2015-12-29 |
EFFECT OF DIETARY ANTIMICROBIAL PEPTIDES-SURFACTIN SUPPLEMENTATION ON PARAMETERS OF INTESTINAL HEALTH INDICES OF GENETICALLY IMPROVED FARMED TILAPIA (GIFT,OREOCHROMIS NILOTICUS) |
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| ZHAI Shao-Wei;SHI Qing-Chao;CHEN Xue-Hao.EFFECT OF DIETARY ANTIMICROBIAL PEPTIDES-SURFACTIN SUPPLEMENTATION ON PARAMETERS OF INTESTINAL HEALTH INDICES OF GENETICALLY IMPROVED FARMED TILAPIA (GIFT,OREOCHROMIS NILOTICUS)[J].Acta Hydrobiologica Sinica,2016,40(4):823-829. |
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| Authors: | ZHAI Shao-Wei;SHI Qing-Chao;CHEN Xue-Hao |
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| Affiliation: | ZHAI Shao-Wei;SHI Qing-Chao;CHEN Xue-Hao;Fisheries College of Jimei Univeristy; |
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| Abstract: | A 7-week feeding trial was conducted to evaluate effects of antimicrobial peptides-surfactin on intestinal health indices of genetically improved farmed tilapia (GIFT, Oreochromis niloticus). Three hundred and twenty fish with initial average weight of (12.01±0.12) g were randomly divided into four treatment groups with four replicates and 20 fish in each replicate. The fish were fed experimental diets supplemented with 0 (control group), 50, 100, and 200 mg/kg antimicrobial peptides-surfactin, respectively. Surfactin supplementation increased intestinal folds height of GIFT (P<0.05), but did not impact the muscular thickness (P>0.05). Surfactin supplementation significantly decreased the number of Escherichia coli of GIFT (P<0.05) and significantly increased the number of Lactobacillus (P<0.05). There was no significant difference in the total bacterial number among all treatment groups (P>0.05). Surfactin supple-mentation significantly increased the total antioxidant capacity level and activities of glutathion peroxidase and super-oxide dismutase (except 200 mg/kg Surfactin supplementation group) (P<0.05) but significantly decreased the content of malondialdehyde (P<0.05). Surfactin supplementation had no effect on catalase activity (P>0.05). The intestinal health parameters of 50 mg/kg antimicrobial peptides-surfactin supplementation group were the best among all supple-mentation groups. In conclusion, surfactin supplementation at 50 mg/kg could improve intestinal health status of GIFT by increasing the intestinal folds height and regulating intestinal microbial population and antioxidant ability. |
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| Keywords: | Antimicrobial peptides Genetically improved farmed tilapia Intestinal health |
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