First Report of Aster Yellows Phytoplasma (16SrI‐B) Associated with Witches' Broom Disease of Melia azedarach var. japonica in Korea |
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| Authors: | Sang‐Sub Han So‐Jin Baek Sang‐Hyun Lee Sang‐Tae Seo Kamala‐Kannan Seralathan |
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| Affiliation: | 1. Department of Forest Environment Science, College of Agriculture and Life Science, Chonbuk National University, Jeonju, Korea;2. Division of Forest Disease and Insect Pest, Korea Forest Research Institute, Seoul, Korea;3. Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Korea |
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| Abstract: | Melia azedarach var. japonica trees with leaf yellowing, small leaves and witches' broom were observed for the first time in Korea. A phytoplasma from the symptomatic leaves was identified based on the 16Sr DNA sequence as a member of aster yellows group, ribosomal subgroup 16SrI‐B. Sequence analyses of more variable regions such as 16S–23S intergenic spacer region, secY gene, ribosomal protein (rp) operon and tuf gene showed 99.5?100% nucleotide identity to several GenBank sequences of group 16SrI phytoplasmas. Phylogenetic analysis confirmed that the Melia azedarach witches' broom phytoplasma belongs to aster yellows group. |
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| Keywords: | Aster yellows phytoplasma Korea
Melia azedarach
secY gene tuf gene |
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