Blastocysts derivation from somatic cell fusion with premature oocytes (prematuration somatic cell fusion) |
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| Authors: | Islam M Saadeldin Candrani Khoirinaya Su Jin Kim Joon Ho Moon Essam Almadaly Byeong Chun Lee |
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| Affiliation: | 1. Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt;2. Department of Theriogenology and Biotechnology, College of Veterinary Medicine and the Research Institute for Veterinary Science, Seoul National University, Seoul, Korea;3. Department of Theriogenology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt;4. Designed Animal and Transplantation, Institute of Green Bio Science Technology, Seoul National University, Seoul, Pyeongchang, Korea |
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| Abstract: | This study was undertaken to investigate the development of immature oocytes after their fusion with male somatic cells expressing red fluorescence protein (RFP). RFP‐expressing cells were fused with immature oocytes, matured in vitro and then parthenogenetically activated. Somatic nuclei showed spindle formation, 1st polar body extrusion after in vitro maturation and protruded the 2nd polar body after parthenogenetic activation. RFP was expressed in the resultant embryos; two‐cell stage and blastocysts. Chromosomal analysis showed aneuploidy in 81.82% of the resulting blastocysts while 18.18% of the resulting blastocysts were diploid. Among eight RFP‐expressing blastocysts, Xist mRNAs was detected in six while Sry mRNA was detected in only one blastocyst. We propose “prematuration somatic cell fusion” as an approach to generate embryos using somatic cells instead of spermatozoa. The current approach, if improved, would assist production of embryos for couples where the male partner is sterile, however, genetic and chromosomal analysis of the resultant embryos are required before transfer to the mothers. |
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| Keywords: | aneuploidy haploidization immature oocyte infertility sterility |
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