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赤眼蜂分子鉴定技术研究
引用本文:李正西,沈佐锐.赤眼蜂分子鉴定技术研究[J].昆虫学报,2002,45(5):559-566.
作者姓名:李正西  沈佐锐
作者单位:中国农业大学昆虫学系,北京,100094
摘    要:通过对6种常见赤眼蜂,即松毛虫赤眼蜂Trichogramma dendrolimi Matsumura、玉米螟赤眼蜂T. ostriniae Pang et Chen、螟黄赤眼蜂T. chilonis Ishii、广赤眼蜂T. evanescens Westwood、甘蓝夜蛾赤眼蜂T. brassicae Bezdenko及食胚赤眼蜂T. embryophagum (Hartig)之核糖体核糖核酸基因第二内部转录区(rDNA-ITS2)的克隆测序,调用GenBank中同源序列,对不同蜂种的rDNA-ITS2序列进行了多重排比和聚类,探讨了rDNA-ITS2用于赤眼蜂属不同种系统进化关系分析及赤眼蜂分子鉴定的可行性。为了考察rDNA-ITS2在赤眼蜂种下水平鉴定上的可能性,作者收集了我国常见的松毛虫赤眼蜂6个地理种群(黑龙江亚布力、吉林长春、吉林仁和、陕西长安、江苏徐州、广东广州),采用相同方法测定了它们的rDNA-ITS2序列。序列分析结果表明,赤眼蜂种下阶元ITS2序列非常保守,而种间存在明显的遗传差异。通过外群比较发现,rDNA-ITS2只适合于赤眼蜂种一级的分子鉴定。

关 键 词:赤眼蜂属  尤氏赤眼蜂属  rDNA  ITS2  分子鉴定  分子系统学  
文章编号:0454-6296(2002)05-0559-08
修稿时间:2002年1月16日

Application of Rdna-ITS2 sequences to the molecular identification of Trichogram-ma spp
LI Zheng Xi,SHEN Zuo Rui.Application of Rdna-ITS2 sequences to the molecular identification of Trichogram-ma spp[J].Acta Entomologica Sinica,2002,45(5):559-566.
Authors:LI Zheng Xi  SHEN Zuo Rui
Affiliation:LI Zheng Xi,SHEN Zuo Rui *
Abstract:The second internal transcribed spacers (ITS2) of six Trichogramma species, i.e. T. dendrolimi Matsumura, T. ostriniae Pang et Chen, T. chilonis Ishii, T. evanescens Westwood, T. brassicae Bezdenko and T. embryophagum (Hartig) were cloned and sequenced, and the homological sequences available in GenBank retrieved for DNA alignment and clustering. The feasibility of the application of ITS2 sequences to the phylogenetic reconstruction and molecular identification of different Trichogramma species was then evaluated. Meanwhile, six geographical populations of T. dendrolimi, i.e., populations collected from Yabuli in Heilongjiang (YBL), Changchun in Jilin (JL), Renhe in Jilin (RH), Chang'an in Shaanxi (CHA), Xuzhou in Jiangsu(XZ) and Guangzhou in Guangdong(GZ), were used to determine ITS2 divergence between subspecies populations. Alignment of sequenced ITS2s showed that they were highly conserved within intraspecific taxa but that significant genetic distances existed between interspecific taxa. Comparison and analysis of an outgroup led to the clear conclusion that ITS2 is suitable only for species-level identification.
Keywords:Trichogramma  Uscana  rDNA  ITS2  molecular identification  molecular systematics
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