| 结合SADF与RAPD标记构建家蚕连锁图 |
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| 引用本文： | 何宁佳,鲁成,李斌,周泽扬,向仲怀.结合SADF与RAPD标记构建家蚕连锁图[J].昆虫学报,2001,44(4):476-482. |
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| 作者姓名： | 何宁佳 鲁成 李斌 周泽扬 向仲怀 |
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| 作者单位： | 1. 西南农业大学蚕桑丝绸学院农业部
2. 湖南师范大学生命科学学院 |
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| 基金项目： | 国家自然科学基金重点资助项目 (39730 370 )，教育部跨世纪优秀青年基金资助项目 |
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| 摘 要： | 用40个选择性扩增多态性引物和137个随机性扩增多态性引物对家蚕Bombyx mori F2群体进行分子标记的筛选。获得544个符合遗传分离比的多态性位点，并用Mapmaker软件进行连锁分析。在最小LOD值为4，最大重组值为0.2条件下拆分连锁群，并对处于同一连锁群的位点进行合理排序，计算出位点间的重组值后转换为图谱距离，构建了一个家蚕的分子连锁图。
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| 关 键 词： | 家蚕 分子标记 连锁图 遗传 |
| 文章编号： | 0454-6296(2001)04-0476-07 |
Construction of a linkage map in the silkworm, Bombyx mori, based on SADF and RAPD markers |
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| HE Ning-jia,LU Cheng,LI Bin,ZHOU Ze-yang,XIANG Zhong-huai.Construction of a linkage map in the silkworm, Bombyx mori, based on SADF and RAPD markers[J].Acta Entomologica Sinica,2001,44(4):476-482. |
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| Authors: | HE Ning-jia LU Cheng LI Bin ZHOU Ze-yang XIANG Zhong-huai |
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| Abstract: | 544 DNA markers which were tested for the expected 3∶1 segregation by the chi-square test for 69 F2 individuals were detected with 40 SADF synthetic primers and 137 RAPD primers. Using these DNA markers the linkage analysis was performed by Mapmaker/Exp (Version 3.0b). The following parameters were preset: two-point analysis (LOD 3.0, maximum recombination value 0.3); three-point and multipoint analysis (LOD 3.0, maximum recombination value no more than 0.2). Linked markers were also identified at the “group” command with LOD score 4 and maximum recombination value 0.2. The functions of Mapmaker were employed to assess the order of the markers in each group. Recombination values were converted into map distance (centimorgan, cM) by applying the Kosambi function. A molecular linkage map of silkworm was constructed. |
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| Keywords: | silkworm DNA markers linkage map genetics |
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