| 家蝇幼虫抗菌相关蛋白/多肽的诱导及抗菌活性分析 |
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| 引用本文： | 安春菊,石明,郝友进,盛长忠,耿华,李德森,杜荣骞.家蝇幼虫抗菌相关蛋白/多肽的诱导及抗菌活性分析[J].昆虫学报,2003,46(5):545-548. |
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| 作者姓名： | 安春菊 石明 郝友进 盛长忠 耿华 李德森 杜荣骞 |
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| 作者单位： | (南开大学生命科学学院, 天津 300071) |
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| 基金项目： | 天津市自然科学基金资助项目 ( 0 3 3 60 4911) |
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| 摘 要： | 对家蝇Musca domestica 3龄幼虫进行针刺、带菌针刺、热激和超声4种处理，并于处理后不同时间分别收集提取家蝇幼虫体内耐热总蛋白，比浊法测定其抗菌活性，经逐步回归分析确定抗菌相关蛋白/多肽。结果表明，4种处理均能诱导家蝇幼虫产生抗菌物质，其中表观分子量为22 kD的蛋白对藤黄微球菌和大肠杆菌均有抗菌作用，50 kD，13 kD，26 kD，7 kD的蛋白抗菌活性具有专一性。还发现一种37 kD的蛋白对抗菌活性有负作用，推测它可能是促进细胞生长的物质。
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| 关 键 词： | 家蝇 诱导 抗菌相关蛋白/多肽 抗菌活性 |
| 文章编号： | 0454-6296(2003)05-0545-04 |
| 修稿时间： | 2002年11月5日 |
Inducement and activity analysis of antibacterial-related proteins/peptides in housefly larvae |
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| AN Chun-Ju,SHI Ming,HAO You-Jin,SHENG Chang-Zhong,GENG Hua,LI De-Sen,DU Rong-Qian.Inducement and activity analysis of antibacterial-related proteins/peptides in housefly larvae[J].Acta Entomologica Sinica,2003,46(5):545-548. |
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| Authors: | AN Chun-Ju SHI Ming HAO You-Jin SHENG Chang-Zhong GENG Hua LI De-Sen DU Rong-Qian |
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| Affiliation: | (College of Life Sciences, Tianjing, 300071) |
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| Abstract: | Third-instar larvae of housefly (Musca domestica) were treated with different inducing methods including pricking, pricking with bacteria, heat shock, and ultrasonication, and collected at different times. The antibacterial activity was measured with liquid phase assay, and the antibacterial-related roteins/peptides were confirmed with stepwise regression model. The results showed that the above four treatments all could induce antibacterial substances. Among these induced proteins, X16 (22 kD) had activity against Micrococcus luteus and Escherichia coli, but X2 (50kD), X12 (26 kD), 22 (13 kD), X24 (7 kD) only had activity against either M. luteus or E. coli, and X7 (37 kD) had negative effect on antibacterial activity, which was deduced to be factors that stimulated cell to grow rapidly. |
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| Keywords: | Musca domestica inducement antibacterial-related proteins/peptides antibacterial activity |
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