| 棉铃虫幼虫脑和咽下神经节的三维结构构建 |
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| 引用本文： | 汤清波,詹欢,Bente G. BERG,闫凤鸣,赵新成.棉铃虫幼虫脑和咽下神经节的三维结构构建[J].昆虫学报,2014,57(5):538-546. |
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| 作者姓名： | 汤清波 詹欢 Bente G. BERG 闫凤鸣 赵新成 |
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| 作者单位： | 河南农业大学植物保护学院昆虫学系;Department |
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| 基金项目： | 国家自然科学基金项目(31272373);公益性行业(农业)科研专项(201203036);河南农业大学博士科研启动基金(30500523) |
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| 摘 要： | 【目的】解剖棉铃虫Helicoverpa armigera (Hübner) 5龄幼虫脑和咽下神经节及其内部神经髓形态结构，并分析和构建幼虫脑和咽下神经节以及各神经髓的三维结构模型。【方法】采用免疫组织化学方法解剖脑和咽下神经节的内部神经髓结构，利用激光共聚焦显微镜获取脑和咽下神经节扫描图像，然后利用AMIRA 三维图像分析软件进行图像分析，从而构建脑和咽下神经节的三维结构模型，并测量脑和咽下神经节以及内部各神经髓的体积，并分析了相对比例。【结果】 棉铃虫5龄幼虫脑和咽下神经节由围咽神经索连接在一起。脑主要由前脑、中脑和后脑3部分组成。前脑内包括视叶、蕈形体和中央体等形态结构较明显的神经髓。此外，前脑还包括其他位于脑的左右两侧以及背侧和腹侧大量神经髓区域，约占脑总神经髓的59.65%。这些神经髓区域边界不明显。中脑主要包括1对触角叶；后脑位于脑的腹侧和触角叶的下方，体积较小。咽下神经节由3个神经节融合构成，从前到后分别为上颚神经节、下颚神经节和下唇神经节，由于融合的紧密程度高，3个神经节间的边界不明显。【结论】阐明了棉铃虫5龄幼虫脑和咽下神经节的神经髓形态结构，构建了脑和咽下神经节以及内部神经髓的三维结构模型。三维模型可以任意旋转，能从任何角度观察脑、咽下神经节和内部不同神经髓的结构及其它们之间的空间关系。本研究结果对研究棉铃虫脑和咽下神经节信息接收、处理及调控行为的机制奠定了解剖学基础。
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| 关 键 词： | 棉铃虫 脑 咽下神经节 神经髓 三维结构 |
Three dimensional reconstructions of the brain and the suboesophageal ganglion of Helicoverpa armigera (Lepidoptera: Noctuidae) larvae |
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| TANG Qing-Bo,ZHAN Huan,Bente G. BERG,YAN Feng-Ming,ZHAO Xin-Cheng.Three dimensional reconstructions of the brain and the suboesophageal ganglion of Helicoverpa armigera (Lepidoptera: Noctuidae) larvae[J].Acta Entomologica Sinica,2014,57(5):538-546. |
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| Authors: | TANG Qing-Bo ZHAN Huan Bente G BERG YAN Feng-Ming ZHAO Xin-Cheng |
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| Abstract: | 【Aim】 This study aims to investigate the neuropil structures of the brain and the suboesophageal ganglion in larvae of the cotton bollworm, Helicoverpa armigera (Hübner) and to reconstruct their three-dimensional models. 【Methods】 The immunohistochemistry was used to characterize the anatomy of the brain and the suboesophageal ganglion. The laser scanning confocal microscope was used to acquire the confocal image stacks of the brain and the suboesophageal ganglion, which were subjected to image analysis, and the digital three-dimensional reconstructions were created by the AMIRA software. The volumes of the brain and the suboesophageal ganglion and their neuropils were measured by using the statistic tool of AMIRA software and the relative size was analyzed. 【Results】 The larval brain and the suboesophageal ganglion are connected by one pair of circumoesophageal connectives. The brain is composed of protocerebrum, deutocerebrum and tritocerebrum. The protocerebrum contains three prominence neuropils, i.e., optic lobes, mushroom bodies and central body. The other neuropils are lateral, ventral and superior protocerebra, which account for 59.65% of the brain neuropils. Their boundary, however, is hard to discriminate. The deutocerebrum mainly consists of a pair of antennal lobes. The tritocerebrum is located in ventral of the brain and under the antennal lobe. Compared with protocerebrum and deutocerebrum, the volume of tritocerebrum is smaller. The suboesophageal ganglion is also a fusion of three neuromeres, i.e., mandibular neuromere, maxillary neuromere, and labial neuromere from anterior to posterior. Their boundaries are obscure. 【Conclusion】 The neuropils of the brain and the suboesophageal ganglion were presented and their digital three-dimensional models were reconstructed. The models can be rotated and viewed at any angle, thus facilitating the identification of the neuropils and their spatial relationships. The results of this study provide knowledge about basic neuroanatomical principles for understanding the mechanisms underlying information processing, integrating and behavioral regulation. |
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| Keywords: | Helicoverpa armigera" target="_blank">Helicoverpa armigera')" href="#">Helicoverpa armigera brain suboesophageal ganglion neuropil three-dimensional model |
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