| 线粒体假基因用作分子化石推算两个榕小蜂姐妹种的分化时间（英文） |
| |
| 引用本文： | 娄文静,滕文佳,刘海洋,李子,肖金花,魏重远,黄大卫.线粒体假基因用作分子化石推算两个榕小蜂姐妹种的分化时间（英文）[J].昆虫学报,2013,56(5):548-553. |
| |
| 作者姓名： | 娄文静 滕文佳 刘海洋 李子 肖金花 魏重远 黄大卫 |
| |
| 作者单位： | （1. 河北大学生命科学学院， 河北保定071002； 2. 中国科学院动物研究所， 中国科学院动物进化与系统学重点实验室， 北京100101） |
| |
| 基金项目： | 国家自然科学基金项目，中国科学院重大创新项目 |
| |
| 摘 要： | 线粒体假基因(nuclear mitochondrial pseudogenes, NUMTs)是指由生物体的线粒体基因组转移至核基因组内的DNA片段。由于其独立进化的特点， NUMTs在用于系统发育分析时是一把双刃剑。我们用基于PCR扩增的方法研究了对叶榕Ficus hispida上两姐妹种榕小蜂Philotrypesis pilosa和Philotrypesis sp.中起源于线粒体Nad1 12S片段的NUMTs。该两姐妹种榕小蜂由同域物种形成过程产生， 它们生活在同一生态环境里（即同一榕果内）， 因此可以用作很好的模型来研究在相同生态环境里物种的行为学及遗传学细微差异的进化。这些深入研究都依赖于对两个物种分化时间的正确估算。通过对所获取的NUMTs进行进化分析， 我们发现： 1）这些NUMTs都是最近引入核基因组事件； 2）NUMTs引入事件发生在物种分化之前。由于这些NUMTs引入核内时间尚短， 其碱基替换速率与线粒体基因相似， 而节肢动物线粒体基因的平均碱基替换速率约为2.3×10-8 替换/位点·年。根据这些进化历史特征可帮助我们将这两个姐妹种榕小蜂的分化时间追溯至0.40-0.48百万年以前。结果提示， 一些线粒体假基因可以很好的用作分子化石来推断一些重要进化事件如物种形成。
|
| 关 键 词： | 榕小蜂 线粒体DNA 线粒体假基因 净化选择 物种分化时间 分子化石 |
Nuclear mitochondrial pseudogenes used as ‘fossil' markers to trace back the speciation time of two sibling fig wasp species |
| |
| LOU Wen-Jing,TENG Wen-Jia,LIU Hai-Yang,LI Zi,XIAO Jin-Hua,WEI Zhong-Yuan,HUANG Da-Wei.Nuclear mitochondrial pseudogenes used as ‘fossil' markers to trace back the speciation time of two sibling fig wasp species[J].Acta Entomologica Sinica,2013,56(5):548-553. |
| |
| Authors: | LOU Wen-Jing TENG Wen-Jia LIU Hai-Yang LI Zi XIAO Jin-Hua WEI Zhong-Yuan HUANG Da-Wei |
| |
| Affiliation: | (1. College of Life Sciences, Hebei University, Baoding, Hebei 071002, China; 2. Key Laboratory of ;
Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China) |
| |
| Abstract: | Nuclear mitochondrial pseudogenes (NUMTs) are DNA fragments transferred from mitochondria to the nucleus. They are a double-edged sword for phylogenetic analyses because of their independent evolutionary histories. Herein, we employed a targeted, PCR-based procedure to study NUMTs originated from a Nad1-12S fragment in two sibling fig wasps associated with Ficus hispida: Philotrypesis pilosa and Philotrypesis sp. These sibling fig wasps arising from sympatric speciation live in the same syconium, which makes them good models to investigate subtle behavioral and genetic divergences in similar ecological niches. Further investigation relies on a correct estimate of the separation time of both species. Through the analysis based on the acquired NUMTs, we found that the origin of the NUMTs in both species appears to be a very recent event; however they may arise in the common ancestor of both species. Since the NUMTs integration is a recent event, we assumed that these newly produced NUMTs evolve similarly to genuine mtDNA. Then we calculated the genetic divergence timing based on mitochondrial genes, an average substitution rate of about 2.3×10-8 substitutions/site/year. These typical characters make them good ‘fossil’ markers to trace back the speciation time of both species back to 0.40-0.48 mya. The results suggest that some NUMTs are good ‘fossil’ markers for tracing back important evolutionary events including speciation. |
| |
| Keywords: | Fig wasp nuclear mitochondrial pseudogene (NUMT) purifying selection mitochondrial DNA speciation timing ‘ fossil’ marker |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《昆虫学报》浏览原始摘要信息 |
|
点击此处可从《昆虫学报》下载全文 |
|
