首页 | 官方网站   微博 | 高级检索  
     

小菜蛾酪氨酸酶生物化学性质研究
引用本文:梁沛,孟凤霞,高希武,郑炳宗,程刚.小菜蛾酪氨酸酶生物化学性质研究[J].昆虫学报,2003,46(5):553-557.
作者姓名:梁沛  孟凤霞  高希武  郑炳宗  程刚
作者单位:中国农业大学昆虫学系,北京,100094
基金项目:国家重大基础研究“973”项目 (G2 0 0 0 0 162 0 7)
摘    要:采用时间动力学法对小菜蛾Plutella xylostella酪氨酸酶的最适反应条件、对抑制剂的敏感度以及发育期变化规律进行了研究。结果表明:小菜蛾酪氨酸酶反应的最适pH值为6.4,反应线性时间为0~2 min,在22℃和4℃下其活性分别可保持12 h 和72 h,在-20℃下至少可保持7 天。不同发育阶段小菜蛾酪氨酸酶活性的大小依次为预蛹>4龄幼虫>蛹>1龄、2龄、3龄幼虫>成虫。苯基硫脲(phenyl thiourea,PTU)对小菜蛾阿维菌素抗性品系和敏感品系酪氨酸酶活性的抑制中浓度(I50)值分别为1.1796 μmol/L和1.2795 μmol/L,二者无明显差异。

关 键 词:小菜蛾    酪氨酸酶    生物化学性质  
文章编号:0454-6296(2003)05-0553-05
修稿时间:2002年12月20

Biochemical characteristics of tyrosinase in the diamondback moth, Plutella xylostella (L.)
LIANG Pei,MENG Feng-Xia,GAO Xi-Wu ,ZHENG Bing-Zong,CHENG Gang.Biochemical characteristics of tyrosinase in the diamondback moth, Plutella xylostella (L.)[J].Acta Entomologica Sinica,2003,46(5):553-557.
Authors:LIANG Pei  MENG Feng-Xia  GAO Xi-Wu  ZHENG Bing-Zong  CHENG Gang
Affiliation:LIANG Pei,MENG Feng-Xia,GAO Xi-Wu *,ZHENG Bing-Zong,CHENG Gang
Abstract:The optimum reaction conditions, sensitivity to the inhibitor and the developmental changes of tyrosinase in the diamondback moth, Plutella xylostella (L.), were studied using time-driving dynamic method. The results showed that the optimum pH of tyrosinase is about 6.4; The OD420 values are linear with time from 0-2 min. When stored at 22℃ and 4℃, the prepared tyrosinase could keep its best activity for 12 h and 72 h, respectively; and while stored at -20℃, the tyrosinase could keep its activity for at least 7 days. The activity of tyrosinase in different developmental stages of P. Xylostella follows the order pharate pupa>4th instar larvae >pupa>1st-3rd instar larvae > adult. The I50 of phenyl thiourea (PTU) to the activity of tyrosinase from both the resistant and susceptible strains showed no obvious difference (1.1796 μmol/L and 1.2795 μmol/L, respectively).
Keywords:Plutella xylostella  tyrosinase  biochemical characteristics
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《昆虫学报》浏览原始摘要信息
点击此处可从《昆虫学报》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司    京ICP备09084417号-23

京公网安备 11010802026262号