| 新疆棉铃虫对溴氰菊酯和硫丹
抗性的生化机理研究 |
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| 引用本文： | 王守玉,谭维嘉,戴小枫,郭予元.新疆棉铃虫对溴氰菊酯和硫丹
抗性的生化机理研究[J].昆虫学报,2000,43(-1):44-50. |
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| 作者姓名： | 王守玉 谭维嘉 戴小枫 郭予元 |
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| 摘 要： | 通过生物测定和生化分析研究了新疆棉铃虫Helicoverpa armigera (Hübner)敏感种群和室内筛选获得的抗性种群对硫丹和溴氰菊酯的反应及其α-乙酸萘酯酶和乙酰胆碱酯酶的动态活性反应。结果表明，筛选后新疆棉铃虫对硫丹和溴氰菊酯产生的抗性倍数分别为13倍和66倍。两个抗性种群的α-乙酸萘酯酶和乙酰胆碱酯酶比活力均高于敏感种群。相应杀虫剂预处理后，α-乙酸萘酯酶酶活力受到抑制。抗性种群的α-乙酸萘酯酶对底物的亲和力高于敏感种群，但Vmax低于敏感种群。抗性种群的乙酰胆碱酯酶对底物的亲和力显著低于敏感种群，Vmax比敏感种群高。聚丙烯酰胺凝胶电泳显示，两个抗性种群都有一条特异性酶带，其迁移率相近，且均可被甲基对氧磷抑制。因此推测，α-乙酸萘酯酶参与了新疆棉铃虫对硫丹和溴氰菊酯的抗性，具有代谢和阻断作用；乙酰胆碱酯酶对抗性的产生也起到了重要作用。
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| 关 键 词： | 棉铃虫 抗性 硫丹 溴氰菊酯 生化机理 |
Biochemical mechanism of resistance to endosulfan
and deltamethrin in cotton bollworm in
Xinjiang Uygur Autonomous Region |
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| WANG Shou-yu,TAN Wei-jia,DAI Xiao-feng,GUO Yu-yuan.Biochemical mechanism of resistance to endosulfan
and deltamethrin in cotton bollworm in
Xinjiang Uygur Autonomous Region[J].Acta Entomologica Sinica,2000,43(-1):44-50. |
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| Authors: | WANG Shou-yu TAN Wei-jia DAI Xiao-feng GUO Yu-yuan |
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| Abstract: | By applying bioassays and biochemical analyses, the reactions to endosulfan and deltamethrin of two cotton bollworm Helicoverpa armigera (Hübner) populations, the susceptible one (collected from Xinjiang) and resistant one (by indoor selection using endosulfan and deltamethrin ),and the activity dynamics of their α-naphthylacetate esterase and acetylcholinesterase (AChE) were tested. The results showed that the resistance ratios to endosulfan and deltamethrin were 13 and 66 folds in Xinjiang cotton bollworm after selection. Α Naphthylacetate esterase and aceylcholinesterase activities of the resistant populations were higher than that of the susceptible. Pretreated with corresponding insecticides the activity of α-naphthylacetate esterase was inhibited. The affinity of α-naphthylacetate esterase to the substrate was higher in the 2 resistant populations than the susceptible, while the maximum velocity (Vmax) was lower. But the affinity of AChE to the substrate was lower and Vmax was higher. It was found on the PAGE profiles that the 2 resistant populations possessed a special band and the immigration rate of this band was similar. In the resistance and susceptible populations the activity of this enzyme band can be inhibited by the inhibitor methyl paraoxon of aceylcholinesterase. These results suggested that α-naphthylacetate esterase had involved in hydrolysis and sequestration and AChE played an important role in the resistance mechanism of cotton bollworm to the 2 insecticides. |
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| Keywords: | cotton bollworm resistance endosulfan deltamethrin biochemical mechanism |
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