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基于16S rDNA序列的柑桔木虱体内共生菌多样性研究
引用本文:殷幼平,刘婷婷,田圣超,胡修峰,吴东,王中康.基于16S rDNA序列的柑桔木虱体内共生菌多样性研究[J].昆虫学报,2011,54(6):664-674.
作者姓名:殷幼平  刘婷婷  田圣超  胡修峰  吴东  王中康
作者单位:1. 重庆大学生物工程学院,重庆市基因功能与调控重点实验室,重庆400030
2. 亚洲果业广西利添生物科技发展,合浦,有限公司,广西北海536128
基金项目:国家自然科学基金项目(309771875); 校企合作科研课题(200706-201312)
摘    要:昆虫消化道内是一个复杂的微生态系统,有大量的微生物存在.这些微生物对寄主发育、营养吸收和防御方面都起着重要的作用.本文利用基于16S rRNA基因的PCR-RFLP指纹图谱法和变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)的方法对柑桔黄龙病虫-媒柑桔木虱Di...

关 键 词:柑桔木虱  共生菌  16S  rDNA  细菌菌群多样性  变性梯度凝胶电泳(DGGE)  RFLP分析
收稿时间:2010-02-01;

Microbial diversity in Diaphorina citri (Homoptera:Psyllidae) estimated by 16S rDNA analysis using DGGE and RFLP
YIN You-Ping,LIU Ting-Ting,TIAN Sheng-Chao,HU Xiu-Feng,WU Dong,WANG Zhong-Kang, Co.Ltd.,Beihai,Guangxi ,China.Microbial diversity in Diaphorina citri (Homoptera:Psyllidae) estimated by 16S rDNA analysis using DGGE and RFLP[J].Acta Entomologica Sinica,2011,54(6):664-674.
Authors:YIN You-Ping  LIU Ting-Ting  TIAN Sheng-Chao  HU Xiu-Feng  WU Dong  WANG Zhong-Kang  CoLtd  Beihai  Guangxi  China
Affiliation:YIN You-Ping1,LIU Ting-Ting1,TIAN Sheng-Chao1,HU Xiu-Feng1,WU Dong2,WANG Zhong-Kang1,(1.Key Lab of Genetic Function and Regulation in Chongqing,College of Bioengineering,Chongqing University,Chongqing 400030,China,2.Lucky Team Biotech Development (Hepu) Co.Ltd.,Beihai,Guangxi 536128,China)
Abstract:The internal gut of insects is a complex micro-ecosystem,in which inhabits a large and varied microbial community.This microbial community plays an important role for their hosts' growth,development,digestion and absorption of nutrition,colonization resistance against invasion of exotic microbes.In this paper,the diversity of bacterial flora of citrus psyllids (Diaphorina citri),which is a vector of the Huanglongbing pathogen Candidatus Liberibacter asiaticus,was investigated based on analysis of 16S rDNA s...
Keywords:Diaphorina citri  endosymbiont bacteria  16S rDNA  microbial diversity  PCR-DGGE  RFLP  
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