| 屋尘螨Ⅰ类变应原Der p1的体内定位 |
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| 引用本文： | 刘志刚,李盟,包莹,付仁龙,苏东明.屋尘螨Ⅰ类变应原Der p1的体内定位[J].昆虫学报,2005,48(6):833-836. |
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| 作者姓名： | 刘志刚 李盟 包莹 付仁龙 苏东明 |
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| 作者单位： | 深圳大学生命科学学院 |
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| 基金项目： | 国家“863”计划项目(2002AA214011)，国家自然科学基金项目(30471505,30271226)，广东省科技重大专项(2003A3080502)，深圳市科技计划资助项目(200218) |
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| 摘 要： | 对过敏性疾病主要过敏原屋尘螨Dermatophagoides pteronyssinusⅠ类变应原（Der p1）进行了抗原定位研究。选用经表达和纯化的Der p1抗原蛋白，按常规方法免疫小鼠，分离血清获抗重组Der p1的抗体（Ⅰ抗），用抗鼠IgG荧光抗体为Ⅱ抗，屋尘螨经石蜡切片，在荧光显微镜下对其特异性变应原的定位进行观察。HE染色显示屋尘螨消化系统占据大部分体腔。组织免疫荧光发现屋尘螨Ⅰ类变应原主要定位于螨的中肠组织及肠内容物，而螨的生殖系统、排泄系统等脏器以及几丁质甲壳均呈阴性反应。据此认为屋尘螨Ⅰ类变应原存在于螨的肠内容物和中肠组织中。
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| 关 键 词： | 屋尘螨 小鼠 Der p1 抗原定位 HE染色 免疫染色 |
| 文章编号： | 0454-6296(2005)06-0833-04 |
| 修稿时间： | 2004年12月8日 |
Antigenic localization of Der p1 in the body of the house dust mite,Dermatophagoides pteronyssinus by immunohistochemistry |
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| LIU Zhi-Gang,LI Meng,BAO Ying,FU Ren-Long,SU Dong-Ming.Antigenic localization of Der p1 in the body of the house dust mite,Dermatophagoides pteronyssinus by immunohistochemistry[J].Acta Entomologica Sinica,2005,48(6):833-836. |
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| Authors: | LIU Zhi-Gang LI Meng BAO Ying FU Ren-Long SU Dong-Ming |
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| Affiliation: | College of Life Sciences， Shenzhen University |
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| Abstract: | The localization of Der p1 in the body of the house dust mite, Dermatophagoides pteronyssinus was studied. Der p1 antibody as primary antibody was prepared by immunizing the mice with Der p1 antigen. The fluorescent antimouse IgG antibody was used as secondary antibody. D. pteronyssinus was embedded in paraffin, sectioned, and then reacted with the primary and secondary antibody. The location of Der p1 in the body was observed with fluoroscope. Some sections were stained with HE staining. A large part of body cavity of D. pteronyssinus was occupied by the digestive system as shown with HE staining. The Der p1 allergen was mostly localized in the midgut and gut contents, while the cuticle and reproductive system showed negative reaction in the immunostaining. |
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| Keywords: | Dermatophagoides pteronyssinus mice Der p1 antigenic localization haematoxylin eosin staining immunostaining |
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