| 高温胁迫下B型烟粉虱热激蛋白基因hsp70表达量的变化 |
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| 引用本文： | 崔旭红,谢明,万方浩.高温胁迫下B型烟粉虱热激蛋白基因hsp70表达量的变化[J].昆虫学报,2007,50(11):1087-1091. |
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| 作者姓名： | 崔旭红 谢明 万方浩 |
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| 作者单位： | 中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京,100094 |
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| 基金项目： | 国家重点基础研究发展规划“973”项目(2002CB111400) |
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| 摘 要： | 为了探讨热激蛋白在B型烟粉虱Bemisia tabaci B-biotype抵抗高温中的作用，以含有B型烟粉虱热激蛋白基因hsp70片段的质粒为模板，用TaqMan-MGB探针和特异性引物构建了B型烟粉虱hsp70实时荧光定量RT-PCR检测体系，并检测了37℃～45℃高温胁迫及气温变化时B型烟粉虱成虫体内hsp70表达的情况。结果表明：缓和的高温对B型烟粉虱成虫hsp70表达具有诱导作用，在37℃～41℃范围内，hsp70的表达量随着温度的升高从8.78×105±6.41×104 拷贝数上升到1.99×107±1.45×105拷贝数，在41℃时达到最高峰；而当温度升高到43℃和45℃，hsp70表达量迅速降低。B型烟粉虱成虫hsp70的表达与昼夜气温变化有关，当上午气温从34℃上升到41℃时，hsp70的表达量从1.16×105±1.48×104拷贝数上升到6.29×106±1.80×105拷贝数；当傍晚气温下降到33℃时，hsp70表达量也下降到2.32×105±7.69×103拷贝数。因此推测，hsp70在B型烟粉虱抵抗高温胁迫方面可能具有重要作用。
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| 关 键 词： | B型烟粉虱 高温胁迫 热激蛋白基因hsp70 表达量 实时荧光定量RT-PCR TaqMan-MGB探针 |
| 文章编号： | 0454-6296（2007）11-1087-05 |
| 收稿时间： | 2007-05-28 |
| 修稿时间： | 2007-09-05 |
Changes in expression level of heat shock protein 70 gene in Bemisia tabaci B-biotype (Homoptera: Aleyrodidae) under high temperature stress |
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| CUI Xu-Hong,XIE Ming,WAN Fang-Hao.Changes in expression level of heat shock protein 70 gene in Bemisia tabaci B-biotype (Homoptera: Aleyrodidae) under high temperature stress[J].Acta Entomologica Sinica,2007,50(11):1087-1091. |
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| Authors: | CUI Xu-Hong XIE Ming WAN Fang-Hao |
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| Abstract: | The plasmid with hsp70 cDNA of Bemisia tabaci B-biotype was used as a template. Based on the GenBank sequence data, one TaqMan-MGB probe and the corresponding primers were designed to construct a standard for real-time fluorescence quantitative RT-PCR to detect the expression of hsp70 in B. tabaci B-biotype, with which the changes in expression level of hsp70 from adults of B. tabaci B-biotype under different high temperature stress from 37℃ to 45℃ were detected. The results showed that mild high temperature could induce hsp70 expression in B. tabaci B-biotype adults. Within the range of 37℃ to 41℃, the hsp70 expression level of B. tabaci B-biotype adults increased from 8.78×105±6.41×104 copies to 1.99×107±1.45×105 copies; However, when the temperature increased to 43℃ and 45℃, the hsp70 expression level decreased very quickly. The hsp70 expression level of B. tabaci B-biotype adults changed in response to the diurnal temperature change. When air temperature increased from 34℃ to 41℃ in the morning, the hsp70 expression level increased dramatically from 1.16×105±1.48×104 copies to 6.29×106±1.80×105 copies; however, the hsp70 expression level decreased to 2.32×105±7.69×103 copies when air temperature decreased to 33℃ at nightfall. It was so inferred that hsp70 in B. tabaci B-biotype may play an important role in high temperature tolerance. |
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| Keywords: | Bemisia tabaci B-biotype high temperature stress hsp70 expression level real-time fluorescence quantitative RT-PCR TaqMan-MGB probe |
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