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| 家蚕AFLP连锁框架图谱的构建 | |
| 引用本文： | 朱玉芳,谭远德,万春玲,鲁成,贺一原,周泽扬,向仲怀.家蚕AFLP连锁框架图谱的构建[J].昆虫学报,2001,44(4):483-493. |
| 作者姓名： | 朱玉芳 谭远德 万春玲 鲁成 贺一原 周泽扬 向仲怀 |
| 作者单位： | 1. 苏州大学生命科学学院生物科学系,南京师范大学生命科学学院, 2. 南京师范大学生命科学学院,湖南师范大学生命科学学院, 3. 南京师范大学生命科学学院, 4. 农业部重点实验室西南农业大学蚕丝学院 5. 中国农业科学院蚕业科学研究所, |
| 基金项目： | 江苏省科委 (A1078)，江苏省教委自然科学基金课题 (2 10 75 )，国家自然科学基金项目 (39870 5 6 8)，国家自然科学基金重点项目 (3970 730 ) |
| 摘 要： | 利用改进的AFLP分子标记方法，对家蚕Bombyx mori回交一代BC1群体进行连锁图谱的构建。经17组AFLP引物的选择性扩增，共得到430个多态位点，卡方检验后有253个为有效位点。利用Mapmaker/Exp (Version 3.0b)软件作连锁分析，其中163个标记分属28个连锁群，连锁群标记数变化范围是2～28个，平均每个连锁群标记数为5.8个，该图覆盖的基因组长度为2.998.9cM(图距单位)，连锁群长度变化范围为4.5～652.8 cM，连锁群的平均长度为107.1 cM，平均图距为4.5～36.7 cM。 |
| 关 键 词： | 家蚕 分子标记 AFLP 连锁图谱 |
| 文章编号： | 0454-6296(2001)04-0483-11 |
Construction of AFLP linkage map of the silkworm (Bombyx mori) |
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| ZHU Yu-fang ,TAN Yuan-de ,WAN Chun-ling ,LU Cheng HE Yi-yuan ,ZHOU Ze-yang ,XIANG Zhong-huai.Construction of AFLP linkage map of the silkworm (Bombyx mori)[J].Acta Entomologica Sinica,2001,44(4):483-493. | |
| Authors: | ZHU Yu-fang TAN Yuan-de WAN Chun-ling LU Cheng HE Yi-yuan ZHOU Ze-yang XIANG Zhong-huai |
| Affiliation: | ZHU Yu-fang 1,2,TAN Yuan-de 2,3,WAN Chun-ling 2,LU Cheng 4 HE Yi-yuan 5,ZHOU Ze-yang 4,XIANG Zhong-huai 4 |
| Abstract: | AFLP markers were mapped using BC1 population ［od100×(782×od100)］. From 47 BC1 samples 430 polymorphic markers were detected using an improved method of AFLP, called silver staining AFLP, which was to some extent different from the original AFLP method, called radiation AFLP. In this study 17 AFLP primer pairs and two restriction enzymes PstⅠ and TaqⅠ were used. Being tested by chi-square test under P=0.05 level for 1∶1 segregation proportion, 253 valid loci that might be used in mapping were obtained. The ratio of the valid loci to polymorphic loci was 58.84%. Twenty eight linkage groups with 163 loci were mapped by using Mapmaker/Exp(Version 3.0b), in which we set LOD=3.0 and maximum recombination fraction= 0.3, and Kosambi function. There were 90 unlinked loci in these 253 valid loci and the ratio of mapping loci to valid loci was 64.43%. The number of loci mapped in the 28 groups was at a range of 2～28 and there were 5.8 (average value) loci per group. The total length of the groups was 2 998.9 cM. The lengths of the 28 groups varied at a range of 4.5～652.8 cM and the average distances between loci on the 28 groups were at a range of 4.5～36.7 cM. The average length of groups was 107.1 cM. |
| Keywords: | silkworm molecular marker AFLP linkage map |
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